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Literature summary for 2.7.1.48 extracted from

  • Murata, D.; Endo, Y.; Obata, T.; Sakamoto, K.; Syouji, Y.; Kadohira, M.; Matsuda, A.; Sasaki, T.
    A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides (2004), Drug Metab. Dispos., 32, 1178-1182.
    View publication on PubMed
Search for more literature for 2.7.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
R162W no activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens
31000
-
 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine Homo sapiens UCK2 enzyme activity required to produce the antitumoral active metabolite ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine Homo sapiens UCK2 enzyme activity required to produce the antitumoral active metabolite ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
ATP + cytidine Homo sapiens rate limiting enzyme of the pyrimidine-nucleotide salvage pathway ADP + CMP
-
 ?
ATP + uridine Homo sapiens rate limiting enzyme of the pyrimidine-nucleotide salvage pathway ADP + UMP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HT-1080 cell fibrosarcoma cell line highly resistant to 3’-ethynyl nucleosides Homo sapiens
-
NUGC-3 cell gastric carcinoma cell line highly resistant to 3’-ethynyl nucleosides Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine
-
 Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine UCK2 enzyme activity required to produce the antitumoral active metabolite Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine 5'-phosphate
-
 ?
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine
-
 Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
ATP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine UCK2 enzyme activity required to produce the antitumoral active metabolite Homo sapiens ADP + 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine 5'-phosphate
-
 ?
ATP + cytidine
-
 Homo sapiens ADP + CMP
-
 ?
ATP + cytidine rate limiting enzyme of the pyrimidine-nucleotide salvage pathway Homo sapiens ADP + CMP
-
 ?
ATP + uridine
-
 Homo sapiens ADP + UMP
-
 ?
ATP + uridine rate limiting enzyme of the pyrimidine-nucleotide salvage pathway Homo sapiens ADP + UMP
-
 ?

Subunits

Subunits Comment Organism
? 1 * 31000, UCK1, SDS-PAGE, 1 * 29000, UCK2, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
More two different enzymes termed UCK1 and UCK2 with 68.8% identity in the deduced amino acid sequence, increased expression of both forms in many tumor cells Homo sapiens