General Stability | Organism |
---|---|
complete loss of activity after storage at -18°C for 24 h in the presence of 0.1 mM dithiothreitol | Mus musculus |
to achieve stability phosphate buffers are used throughout purification and for storage of enzyme fractions | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 13 mM, 66% of activity with Mg2+ | Mus musculus | |
Fe2+ | can partially replace Mg2+ | Mus musculus | |
Fe2+ | 13 mM, 65% of activity with Mg2+ | Mus musculus | |
Mg2+ | required for full activity | Mus musculus | |
Mg2+ | maximal activity at 13 mM | Mus musculus | |
Mn2+ | can partially replace Mg2+ | Mus musculus | |
Mn2+ | 13 mM, 80% of activity with Mg2+ | Mus musculus | |
additional information | not activated by Ca2+ and Cu2+ | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, ammonium sulfate, hydroxyapatite | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mastocytoma cell | P815 cell line | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.03 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-18°C, phosphate buffer, pH 7.4-8.2, several months, no loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-azacytidine | 39.1% of the activity with uridine | Mus musculus | ADP + 5-azacytidine 5'-monophosphate | - |
? | |
ATP + 5-fluorouridine | 66.9% of the activity with uridine | Mus musculus | ADP + 5-fluorouridine 5'-monophosphate | - |
? | |
ATP + 6-azacytidine | 60.4% of the activity with uridine | Mus musculus | ADP + 6-azacytidine 5'-monophosphate | - |
? | |
ATP + 6-azauridine | 65.5% of the activity with uridine | Mus musculus | ADP + 6-azauridine 5'-monophosphate | - |
? | |
ATP + cytidine | 50% of activity with uridine | Mus musculus | ADP + CMP | - |
? | |
ATP + cytidine | 35.6% of the activity with uridine | Mus musculus | ADP + CMP | - |
? | |
ATP + uridine | no activity with thymine riboside, deoxyribosides, cytosine arabinoside, adenosine, purine deoxyribosides, UMP, CMP and deoxyribosides | Mus musculus | ADP + UMP | - |
? | |
ATP + uridine | ATP most effective as phosphate donor | Mus musculus | ADP + UMP | - |
? | |
dATP + uridine | 20.5% of activity with ATP | Mus musculus | dADP + UMP | - |
? | |
dCTP + uridine | 49.7% of activity with ATP | Mus musculus | dCDP + UMP | - |
? | |
dGTP + uridine | 23.5% of activity with ATP | Mus musculus | dGDP + UMP | - |
? | |
dUTP + uridine | 66.7% of activity with ATP | Mus musculus | dUDP + UMP | - |
? | |
GTP + uridine | - |
Mus musculus | UMP + GDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Mus musculus |