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Literature summary for 2.7.1.47 extracted from
- D-Ribulokinase from Aerobacter aerogenes (1959), J. Biol. Chem., 234, 3097-3101.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activation | Klebsiella aerogenes |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3 | Klebsiella aerogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
ATP | pH 7.7, 27°C | Klebsiella aerogenes | |
| 0.92 | - |
D-ribulose | pH 7.7, 37°C | Klebsiella aerogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, can replace Mg2+ with 23% efficiency | Klebsiella aerogenes | |
| Mg2+ | optimum Mg/ATP-ratio: 1-2, inhibits at higher values | Klebsiella aerogenes | |
| Mn2+ | activation, can replace Mg2+ with 54% efficiency | Klebsiella aerogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | Klebsiella aerogenes | inducible enzyme of ribitol catabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | specific for D-isomer | Klebsiella aerogenes | ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + D-ribulose | ATP cannot be replaced by ITP, GTP or ADP | Klebsiella aerogenes | ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + D-ribulose | inducible enzyme of ribitol catabolism | Klebsiella aerogenes | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | broad | Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5 | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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