General Stability | Organism |
---|---|
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity | Vigna radiata var. radiata |
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity | Pisum sativum |
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity | Petroselinum crispum |
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity | Raphanus sativus |
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity | Fagopyrum esculentum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent metal ion is required, lower activity than Mg2+ or Mn2+ | Vigna radiata var. radiata | |
Co2+ | divalent metal ion is required, lower activity than Mg2+ or Mn2+ | Pisum sativum | |
Co2+ | divalent metal ion is required, lower activity than Mg2+ or Mn2+ | Petroselinum crispum | |
Co2+ | divalent metal ion is required, lower activity than Mg2+ or Mn2+ | Raphanus sativus | |
Co2+ | divalent metal ion is required, lower activity than Mg2+ or Mn2+ | Fagopyrum esculentum | |
Mg2+ | divalent metal ion is required, Zn2+ or Ni2+ are ineffective | Vigna radiata var. radiata | |
Mg2+ | divalent metal ion is required, Zn2+ or Ni2+ are ineffective | Pisum sativum | |
Mg2+ | divalent metal ion is required, Zn2+ or Ni2+ are ineffective | Petroselinum crispum | |
Mg2+ | divalent metal ion is required, Zn2+ or Ni2+ are ineffective | Raphanus sativus | |
Mg2+ | divalent metal ion is required, Zn2+ or Ni2+ are ineffective | Fagopyrum esculentum | |
Mn2+ | divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective | Vigna radiata var. radiata | |
Mn2+ | divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective | Pisum sativum | |
Mn2+ | divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective | Petroselinum crispum | |
Mn2+ | divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective | Raphanus sativus | |
Mn2+ | divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective | Fagopyrum esculentum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-galacturonate | Vigna radiata var. radiata | involved in formation of pectin | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | Pisum sativum | involved in formation of pectin | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | Petroselinum crispum | involved in formation of pectin | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | Raphanus sativus | involved in formation of pectin | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | Fagopyrum esculentum | involved in formation of pectin | ADP + 1-phospho-alpha-D-galacturonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fagopyrum esculentum | - |
buckwheat | - |
Petroselinum crispum | - |
parsley | - |
Pisum sativum | - |
pea | - |
Raphanus sativus | - |
radish | - |
Vigna radiata var. radiata | - |
mung bean | - |
Purification (Comment) | Organism |
---|---|
partial | Vigna radiata var. radiata |
partial | Pisum sativum |
partial | Petroselinum crispum |
partial | Raphanus sativus |
partial | Fagopyrum esculentum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Petroselinum crispum | - |
root | - |
Raphanus sativus | - |
seed | germinating | Vigna radiata var. radiata | - |
seed | germinating | Pisum sativum | - |
seed | germinating | Fagopyrum esculentum | - |
Storage Stability | Organism |
---|---|
4°C, 0.05 M mercaptoethanol, stable up to 10 days | Vigna radiata var. radiata |
4°C, 0.05 M mercaptoethanol, stable up to 10 days | Pisum sativum |
4°C, 0.05 M mercaptoethanol, stable up to 10 days | Petroselinum crispum |
4°C, 0.05 M mercaptoethanol, stable up to 10 days | Raphanus sativus |
4°C, 0.05 M mercaptoethanol, stable up to 10 days | Fagopyrum esculentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-galacturonate | - |
Vigna radiata var. radiata | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | - |
Pisum sativum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | - |
Petroselinum crispum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | - |
Raphanus sativus | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | - |
Fagopyrum esculentum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | involved in formation of pectin | Vigna radiata var. radiata | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | involved in formation of pectin | Pisum sativum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | involved in formation of pectin | Petroselinum crispum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | involved in formation of pectin | Raphanus sativus | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
ATP + D-galacturonate | involved in formation of pectin | Fagopyrum esculentum | ADP + 1-phospho-alpha-D-galacturonate | - |
? | |
additional information | D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides | Vigna radiata var. radiata | ? | - |
? | |
additional information | D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides | Pisum sativum | ? | - |
? | |
additional information | D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides | Petroselinum crispum | ? | - |
? | |
additional information | D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides | Raphanus sativus | ? | - |
? | |
additional information | D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides | Fagopyrum esculentum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vigna radiata var. radiata |
37 | - |
assay at | Pisum sativum |
37 | - |
assay at | Petroselinum crispum |
37 | - |
assay at | Raphanus sativus |
37 | - |
assay at | Fagopyrum esculentum |