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Literature summary for 2.7.1.40 extracted from
- Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | product inhibition. Inhibition cannot be reversed by presence of phophoenolpyruvate or ADP | Saccharolobus solfataricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.093 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.113 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.115 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.137 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.164 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.225 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 0.26 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49800 | - |
x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
| 50000 | - |
x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97ZD7 | - |
- |
| Saccharolobus solfataricus DSM 1617 | Q97ZD7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Saccharolobus solfataricus | ATP + pyruvate | - |
r | |
| ADP + phosphoenolpyruvate | - |
Saccharolobus solfataricus DSM 1617 | ATP + pyruvate | - |
r | |
| ATP + pyruvate | - |
Saccharolobus solfataricus | ADP + phosphoenolpyruvate | - |
r | |
| ATP + pyruvate | - |
Saccharolobus solfataricus DSM 1617 | ADP + phosphoenolpyruvate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SSO0981 | - |
Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 39.67 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 40.06 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 50.12 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 52.85 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 58.4 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 58.78 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 73.04 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 80.29 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
65°C, pH not specified in the publication | Saccharolobus solfataricus | ATP | |
| 0.8 | - |
70°C, pH not specified in the publication | Saccharolobus solfataricus | ATP | |
| 0.8 | - |
80°C, pH not specified in the publication | Saccharolobus solfataricus | ATP | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 137.3 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 244.3 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 280.9 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 385.8 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 435.8 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 628 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 710.5 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
| 851.9 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
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