Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | phosphorylation of serine and threonine residues is, besides being essential for isozyme M2 catalytic activity, induces a trimeric association of the ProTalpha kinase. This association can be shifted to a tetrameric form by fructose 1,6-bisphosphate, which results in a decrease in ProTalphaK activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + prothymosin alpha | Homo sapiens | - |
ADP + phospho-prothymosin alpha | - |
? | |
ATP + pyruvate | Homo sapiens | - |
ADP + phosphoenolpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
M2 isoform | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of serine and threonine residues is, besides being essential for isozyme M2 catalytic activity, induces a trimeric association of the ProTalpha kinase. This association can be shifted to a tetrameric form by fructose 1,6-bisphosphate, which results in a decrease in ProTalphaK activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
native enzyme from cytosol by ammonium sulfate fractionation and prothymosin alpha affinity and ion exchange chromatography, and chromatography on a phosphocellulose resin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte | M2-type isoenzyme | Homo sapiens | - |
NC-37 cell | a lymphoblastoid cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + prothymosin alpha | - |
Homo sapiens | ADP + phospho-prothymosin alpha | - |
? | |
ATP + prothymosin alpha | isozyme M2-type acts as a ProTalpha kinase phosphorylating ProTalpha | Homo sapiens | ADP + phospho-prothymosin alpha | - |
? | |
ATP + pyruvate | - |
Homo sapiens | ADP + phosphoenolpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ProTalphaK | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | acquisition of ProTalpha kinase activity by M2 isozyme seems to be due to the phosphorylation of serine and threonine residues, which, besides being essential for its catalytic activity, induces a trimeric association of ProTalpha kinase. Cytosolic phosphorylation of ProTaalpha, which then migrates to the nucleus, where it influences chromatin activity | Homo sapiens |