Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | allosteric stimulation | Leishmania donovani | |
additional information | no stimulation: fructose 1,6-bisphosphate | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
- |
Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
H480Q | site-directed mutagenesis of putative binding site of fructose 2,6-bisphosphate. Mutant displays hyperbolic kinetics that is not changed by addition of the allosteric effector fructose 2,6-bisphosphate | Leishmania donovani |
K453L | site-directed mutagenesis of putative binding site of fructose 2,6-bisphosphate. Mutant retains a sigmoidal kinetics and is little affected by addition of fructose 2,6-bisphosphate | Leishmania donovani |
K453L/H480Q | site-directed mutagenesis of putative binding sites of fructose 2,6-bisphosphate. Mutant displays hyperbolic kinetics | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in the presence of the effector fructose 2,6-bisphosphate the enzyme displays a hyperbolic saturation curve, whereas in the absence of fructose 2,6-bisphosphate the curve is sigmoid. The phosphoenolpyruvate concentration for S0.5 in presence of fructose 2,6-bisphosphate is 0.08 mM, and 1.16 mM in absence, with a concomitant increase in Vmax from 492.1 U/mg in absence to 506.99 U/mg in presence of fructose 2,6-bisphosphate. For ADP, a Km of 0.32 mM has been calculated | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54400 | - |
x * 54400, calculated | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | A7LIU4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
68.2 | - |
mutant H480Q, pH 7.2, 25°C | Leishmania donovani |
98 | - |
mutant K453L/H480Q, pH 7.2, 25°C | Leishmania donovani |
156.9 | - |
mutant K453L, pH 7.2, 25°C | Leishmania donovani |
178.1 | - |
wild-type, pH 7.2, 25°C | Leishmania donovani |
Subunits | Comment | Organism |
---|---|---|
? | x * 54400, calculated | Leishmania donovani |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania donovani | calulated | - |
6.5 |