Literature summary for 2.7.1.40 extracted from

Plaxton, W.C.; Smith, C.R.; Knowles, V.L.
Molecular and regulatory properties of leucoplast pyruvate kinase from Brassica napus (rapeseed) suspension cells (2002), Arch. Biochem. Biophys., 400, 54-62.

Search for more literature for 2.7.1.40

Activating Compound

Activating Compound	Comment	Organism	Structure
6-phosphogluconate	at 1 mM, 200% of activity	Brassica napus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxoglutarate	50% inhibition at 8.3 mM	Brassica napus
citrate	50% inhibition at 18.4 mM	Brassica napus
oxalate	50% inhibition at 0.23 mM	Brassica napus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.052	-	phosphoenolpyruvate	24°C, pH 8.0	Brassica napus
0.14	-	ADP	pH 8.0, 24°C	Brassica napus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	3 * 58000 + 3 64000, SDS-PAGE	Brassica napus
380000	-	gel filtration	Brassica napus

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	-	PKp, plastidic form	-

Purification (Commentary)

Purification (Comment)	Organism
-	Brassica napus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate	-	Brassica napus	ATP + pyruvate	-	?

Subunits

Subunits	Comment	Organism
hexamer	3 * 58000 + 3 64000, SDS-PAGE	Brassica napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Brassica napus

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Release 2023.1 (January 2023)