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Literature summary for 2.7.1.40 extracted from
- Purification and properties of the pyruvate kinase isozyme M1 from the pig brain (1995), Int. J. Biochem. Cell Biol., 27, 1145-1151.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | not | Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE, M1 isozyme | Sus scrofa |
| 230000 | - |
sucrose density gradient centrifugation, M1 isozyme | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
M1 isozyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate = ADP + phosphoenolpyruvate | hyperbolic kinetics | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 191 | - |
pH 7.4, 22°C | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Sus scrofa | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE, M1 isozyme | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Sus scrofa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sus scrofa | isoelectric focusing | - |
8 |
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