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Literature summary for 2.7.1.40 extracted from
- Purification and characterization of cytosolic pyruvate kinase from Brassica napus (rapeseed) suspension cell cultures: implications for the integration of glycolysis with nitrogen assimilation (2000), Eur. J. Biochem., 267, 4477-4485.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-aspartate | Ka-value 0.31 mM, reverses inhibition by L-glutamate | Brassica napus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-glutamate | 50% inhibition at 4 mM | Brassica napus | |
| oxalate | 50% inhibition at 4 mM | Brassica napus | |
| quercetin | 50% inhibition at 0.1 mM | Brassica napus | |
| rutin | 50% inhibition at 0.07 mM | Brassica napus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | hyperbolic saturation kinetics for phosphoenolpyruvate, ADP, Mg2+, K+ | Brassica napus | |
| 0.075 | - |
ADP | pH 6.8, 24°C | Brassica napus | |
| 0.12 | - |
phosphoenolpyruvate | pH 6.8, 24°C | Brassica napus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Brassica napus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | apparent Km-value: 0.48 mM | Brassica napus | |
| K+ | only together with activating divalent cation | Brassica napus | |
| Mg2+ | apparent Km-value: 0,21 mM | Brassica napus | |
| Mg2+ | fulfills absolute requirement for divalent cation | Brassica napus | |
| additional information | absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this | Brassica napus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
4 * 56000, SDS-PAGE | Brassica napus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
PKc, cytosolic form | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica napus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51 | - |
pH 6.8, 24°C | Brassica napus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Brassica napus | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 56000, SDS-PAGE | Brassica napus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
3 min, stable | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
broad | Brassica napus |
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Release 2023.1 (January 2023)
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