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Literature summary for 2.7.1.40 extracted from

  • Ireland, R.J.; De Luca, V.; Dennis, D.T.
    Characterization and kinetics of isoenzymes of pyruvate kinase from developing castor bean endosperm (1980), Plant Physiol., 65, 1188-1193.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.40

General Stability

General Stability Organism
cytosolic isozyme is much more stable than plastid isozyme, dithiothreitol plus glycerol stabilize plastid isozyme PKp during purification, isozyme PKp requires dithiothreitol or 2-mercaptoethanol during purification, cytosolic isozyme PKc is stable to dilution into 50 mM N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9 Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Ricinus communis
pyruvate product inhibition Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters Ricinus communis
0.03 0.05 ADP 25°C, pH 7.9 Ricinus communis
0.04 0.063 phosphoenolpyruvate 25°C, pH 7.9 Ricinus communis
0.04 0.063 phosphoenolpyruvate 2 isozymes with different kinetic mechanisms Ricinus communis
0.049 0.1 IDP 25°C, pH 7.9 Ricinus communis
0.049 0.1 IDP cosubstrate phosphoenolpyruvate Ricinus communis
0.12
-
 ADP cosubstrate UDP, isozyme PKc, 25°C, pH 7.9 Ricinus communis
0.2 0.35 ADP 25°C, pH 7.9 Ricinus communis
0.2 0.35 ADP PKp-isozyme Ricinus communis
0.25 0.26 GDP IDP, isozyme PKc, 25°C, pH 7.9 Ricinus communis
2.2
-
 GDP isozyme PKp, 25°C, pH 7.9 Ricinus communis
2.4
-
 UDP isozyme PKp, 25°C, pH 7.9 Ricinus communis
4
-
 IDP isozyme PKp, 25°C, pH 7.9 Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 2 isozymes: a cytosolic and a plastid one Ricinus communis 5829
-
plastid 2 isozymes: a cytosolic and a plastid one Ricinus communis 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ fulfills requirement for monovalent cation Ricinus communis
Mg2+ requirement Ricinus communis
Mg2+ Km-value 0.45 mM for isozyme PKc, 1.6 mM for isozyme PKp Ricinus communis
Mg2+ fulfills absolute requirement for divalent cation Ricinus communis
Mn2+ requirement Ricinus communis
Mn2+ fulfills absolute requirement for divalent cation Ricinus communis
Mn2+ Km-value 0.05 mM for isozyme PKc, 0.5 mM for isozyme PKp Ricinus communis
NH4+ requirement Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
 castor bean, var. Baker 296
-

Purification (Commentary)

Purification (Comment) Organism
partial, several isozymes Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
endosperm developing Ricinus communis
-
seed
-
 Ricinus communis
-

Storage Stability

Storage Stability Organism
5°C, isozyme PKp in 50 mM phosphate buffer, pH 7, 50%, v/v, glycerol, 2 mM dithiothreitol, several months and isozyme PKc in 50 mM phosphate buffer, pH 7, at least 1 week Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Ricinus communis ATP + pyruvate
-
 ?
CDP + phosphoenolpyruvate less effective than ADP Ricinus communis CTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Ricinus communis GTP + pyruvate
-
 ?
IDP + phosphoenolpyruvate less effective than ADP Ricinus communis ITP + pyruvate
-
 ?
UDP + phosphoenolpyruvate less effective than ADP Ricinus communis UTP + pyruvate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ricinus communis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 in N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9, 1% glycerol, 0.04 mM DTT, isozyme PKp: t1/2: 1.5 min, ADP and MgCl2 preserve activity over 10 min, isozyme PKc: at least 30 min Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.2 isozyme PKc Ricinus communis
7.8
-
 isozyme PKp Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
6 8.3 isozyme PKc, about half-maximal activity at pH 6 and pH 8.3 Ricinus communis
7.2 8.3 isozyme Pkp, about half-maximal activity at pH 7.2 and pH 8.3 Ricinus communis