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Literature summary for 2.7.1.4 extracted from
- Properties of recombinant ATP-dependent fructokinase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2012), Biochemistry, 77, 372-377.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Methylotuvimicrobium alcaliphilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Methylotuvimicrobium alcaliphilum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
D-fructose | pH 9, 30°C | Methylotuvimicrobium alcaliphilum | |
| 1.3 | - |
ATP | pH 9, 30°C | Methylotuvimicrobium alcaliphilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required for optimal activity | Methylotuvimicrobium alcaliphilum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylotuvimicrobium alcaliphilum | G4T023 | - |
- |
| Methylotuvimicrobium alcaliphilum DSM 19304 | G4T023 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using metal chelate affinity chromatography | Methylotuvimicrobium alcaliphilum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.2 mM imidazole buffer (pH 8.0), two months | Methylotuvimicrobium alcaliphilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Methylotuvimicrobium alcaliphilum | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-fructose | - |
Methylotuvimicrobium alcaliphilum DSM 19304 | ADP + D-fructose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FRK | - |
Methylotuvimicrobium alcaliphilum |
| MEALZv4_0616 | - |
Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Methylotuvimicrobium alcaliphilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
after 1 h the residual activity is 80% | Methylotuvimicrobium alcaliphilum |
| 50 | - |
after 5 min the residual activity is 20% | Methylotuvimicrobium alcaliphilum |
| 60 | - |
after 30 min the enzymatic activity is completely lost | Methylotuvimicrobium alcaliphilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Methylotuvimicrobium alcaliphilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Methylotuvimicrobium alcaliphilum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
ADP | pH 9, 30°C | Methylotuvimicrobium alcaliphilum | |
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