Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | isoform FRK2, above 0.1 mM, with substrate ATP | Saccharum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
D-fructose | isoform FRK1, pH 8.0, 25°C | Saccharum sp. | |
0.074 | - |
D-fructose | isoform FRK2, pH 8.0, 25°C | Saccharum sp. | |
0.14 | - |
ATP | isoform FRK1, pH 8.0, 25°C | Saccharum sp. | |
0.18 | - |
ATP | isoform FRK2, pH 8.0, 25°C | Saccharum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2 | Saccharum sp. |
69000 | - |
gel filtration, both isoform FRK1 and FRK2 | Saccharum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharum sp. | - |
var N19, isoforms FRK1 and FRK2 | - |
Purification (Comment) | Organism |
---|---|
isoforms FRK1 and FRK2 | Saccharum sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
internode | - |
Saccharum sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.83 | - |
isoform FRK1, pH 8.0, 25°C | Saccharum sp. |
44.05 | - |
isoform FRK1, pH 8.0, 25°C | Saccharum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Saccharum sp. | ADP + D-fructose 6-phosphate | - |
? | |
CTP + D-fructose | isoform FRK1, 35% of activity with ATP, isoform FRK2, 83% of activity with ATP | Saccharum sp. | CDP + D-fructose 6-phosphate | - |
? | |
GTP + D-fructose | isoform FRK1, 23% of activity with ATP, isoform FRK2, 135% of activity with ATP | Saccharum sp. | GDP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | isoform FRK1, 39% of activity with ATP, isoform FRK2, 84% of activity with ATP | Saccharum sp. | UDP + D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2 | Saccharum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isoforms FRK1 and FRK2 | Saccharum sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
30% loss of activity, both isoform FRK1 and FRK2 | Saccharum sp. |
7.5 | 9 | both isoform FRK1 and FRK2 | Saccharum sp. |
10 | - |
40% loss of activity, both isoform FRK1 and FRK2 | Saccharum sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0161 | - |
D-fructose | isoform FRK2, pH 8.0, 25°C | Saccharum sp. |