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Literature summary for 2.7.1.4 extracted from

  • Hoepfner, S.W.; Botha, F.C.
    Purification and characterisation of fructokinase from the culm of sugarcane. (2004), Plant Sci., 167, 645-654.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-fructose isoform FRK2, above 0.1 mM, with substrate ATP Saccharum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
D-fructose isoform FRK1, pH 8.0, 25°C Saccharum sp.
0.074
-
D-fructose isoform FRK2, pH 8.0, 25°C Saccharum sp.
0.14
-
ATP isoform FRK1, pH 8.0, 25°C Saccharum sp.
0.18
-
ATP isoform FRK2, pH 8.0, 25°C Saccharum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2 Saccharum sp.
69000
-
gel filtration, both isoform FRK1 and FRK2 Saccharum sp.

Organism

Organism UniProt Comment Textmining
Saccharum sp.
-
var N19, isoforms FRK1 and FRK2
-

Purification (Commentary)

Purification (Comment) Organism
isoforms FRK1 and FRK2 Saccharum sp.

Source Tissue

Source Tissue Comment Organism Textmining
internode
-
Saccharum sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.83
-
isoform FRK1, pH 8.0, 25°C Saccharum sp.
44.05
-
isoform FRK1, pH 8.0, 25°C Saccharum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Saccharum sp. ADP + D-fructose 6-phosphate
-
?
CTP + D-fructose isoform FRK1, 35% of activity with ATP, isoform FRK2, 83% of activity with ATP Saccharum sp. CDP + D-fructose 6-phosphate
-
?
GTP + D-fructose isoform FRK1, 23% of activity with ATP, isoform FRK2, 135% of activity with ATP Saccharum sp. GDP + D-fructose 6-phosphate
-
?
UTP + D-fructose isoform FRK1, 39% of activity with ATP, isoform FRK2, 84% of activity with ATP Saccharum sp. UDP + D-fructose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2 Saccharum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
isoforms FRK1 and FRK2 Saccharum sp.

pH Range

pH Minimum pH Maximum Comment Organism
6
-
30% loss of activity, both isoform FRK1 and FRK2 Saccharum sp.
7.5 9 both isoform FRK1 and FRK2 Saccharum sp.
10
-
40% loss of activity, both isoform FRK1 and FRK2 Saccharum sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0161
-
D-fructose isoform FRK2, pH 8.0, 25°C Saccharum sp.