KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | pH 7.6, 45°C | Pseudomonas sp. | |
0.7 | - |
fructose | pH 7.6, 45°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas sp. | |
Mn2+ | can partially replace Mg2+ in activation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Pseudomonas sp. |
75000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Pseudomonas sp. | - |
ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | Pseudomonas sp. KN-21 | - |
ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
KN-21 | - |
Pseudomonas sp. KN-21 | - |
KN-21 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
5°C, stable for 6 weeks at pH 8 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Pseudomonas sp. | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | - |
Pseudomonas sp. KN-21 | ADP + D-fructose 6-phosphate | - |
? | |
CTP + D-fructose | 40% of the activity with ATP | Pseudomonas sp. | CDP + D-fructose 6-phosphate | - |
? | |
dATP + D-fructose | 90% of the activity with ATP | Pseudomonas sp. | dADP + D-fructose 6-phosphate | - |
? | |
dATP + D-fructose | 90% of the activity with ATP | Pseudomonas sp. KN-21 | dADP + D-fructose 6-phosphate | - |
? | |
GTP + D-fructose | 30% of the activity with ATP | Pseudomonas sp. | GDP + D-fructose 6-phosphate | - |
? | |
GTP + D-fructose | 30% of the activity with ATP | Pseudomonas sp. KN-21 | GDP + D-fructose 6-phosphate | - |
? | |
ITP + D-fructose | 25% of the activity with ATP | Pseudomonas sp. | IDP + D-fructose 6-phosphate | - |
? | |
ITP + D-fructose | 25% of the activity with ATP | Pseudomonas sp. KN-21 | IDP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | 80% of the activity with ATP | Pseudomonas sp. | UDP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | 80% of the activity with ATP | Pseudomonas sp. KN-21 | UDP + D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | stable | Pseudomonas sp. |