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Literature summary for 2.7.1.4 extracted from

  • Murao, S.; Nakatani, A.; Kaneda, N.
    Isolation and characterization of fructokinase from Pseudomonas sp. KN-21 (1995), Biosci. Biotechnol. Biochem., 59, 1798-1800.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
ATP pH 7.6, 45°C Pseudomonas sp.
0.7
-
fructose pH 7.6, 45°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas sp.
Mn2+ can partially replace Mg2+ in activation Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Pseudomonas sp.
75000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Pseudomonas sp.
-
ADP + D-fructose 6-phosphate
-
?
ATP + D-fructose Pseudomonas sp. KN-21
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
KN-21
-
Pseudomonas sp. KN-21
-
KN-21
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
5°C, stable for 6 weeks at pH 8 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Pseudomonas sp. ADP + D-fructose 6-phosphate
-
?
ATP + D-fructose
-
Pseudomonas sp. KN-21 ADP + D-fructose 6-phosphate
-
?
CTP + D-fructose 40% of the activity with ATP Pseudomonas sp. CDP + D-fructose 6-phosphate
-
?
dATP + D-fructose 90% of the activity with ATP Pseudomonas sp. dADP + D-fructose 6-phosphate
-
?
dATP + D-fructose 90% of the activity with ATP Pseudomonas sp. KN-21 dADP + D-fructose 6-phosphate
-
?
GTP + D-fructose 30% of the activity with ATP Pseudomonas sp. GDP + D-fructose 6-phosphate
-
?
GTP + D-fructose 30% of the activity with ATP Pseudomonas sp. KN-21 GDP + D-fructose 6-phosphate
-
?
ITP + D-fructose 25% of the activity with ATP Pseudomonas sp. IDP + D-fructose 6-phosphate
-
?
ITP + D-fructose 25% of the activity with ATP Pseudomonas sp. KN-21 IDP + D-fructose 6-phosphate
-
?
UTP + D-fructose 80% of the activity with ATP Pseudomonas sp. UDP + D-fructose 6-phosphate
-
?
UTP + D-fructose 80% of the activity with ATP Pseudomonas sp. KN-21 UDP + D-fructose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 stable Pseudomonas sp.