Literature summary for 2.7.1.4 extracted from

Martinez-Barajas, E.; Krohn, B.M.; Stark, D.M.; Randall, D.D.
Purification and characterization of recombinant tomato fruit (Lycopersicon esculentum Mill.) fructokinase expressed in Escherichia coli (1997), Protein Expr. Purif., 11, 41-46.

Search for more literature for 2.7.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
-	Solanum lycopersicum

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	inhibition is dependent on fructose concentration	Solanum lycopersicum
D-fructose	above 5 mM	Solanum lycopersicum
D-fructose 6-phosphate	weak, non-competitive	Solanum lycopersicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13	-	ATP	recombinant enzyme, pH 8, 25°C	Solanum lycopersicum
0.22	-	D-fructose	recombinant enzyme, pH 8, 25°C	Solanum lycopersicum
0.45	-	GTP	recombinant enzyme, pH 8, 25°C	Solanum lycopersicum
1.86	-	UTP	recombinant enzyme, pH 8, 25°C	Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	2 * 35000, SDS-PAGE	Solanum lycopersicum
72400	-	gel filtration	Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Solanum lycopersicum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
22.59	-	-	Solanum lycopersicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose	-	Solanum lycopersicum	ADP + D-fructose 6-phosphate	-	?
GTP + D-fructose	-	Solanum lycopersicum	GDP + D-fructose 6-phosphate	-	?
UTP + D-fructose	-	Solanum lycopersicum	UDP + D-fructose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE	Solanum lycopersicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Solanum lycopersicum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.12	-	ADP	at 3 mM fructose, pH 8, 25°C	Solanum lycopersicum
0.41	-	ADP	at 0.5 mM fructose, pH 8, 25°C	Solanum lycopersicum
3	-	D-fructose	pH 8, 25°C	Solanum lycopersicum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Solanum lycopersicum	six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE	6.17	5.66

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Release 2023.1 (January 2023)