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Literature summary for 2.7.1.4 extracted from

  • Doehlert, D.C.
    Separation and characterization of four hexose kinases from developing maize kernels (1989), Plant Physiol., 89, 1042-1048.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Zea mays
D-fructose
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
D-fructose 30°C, pH 8.5 Zea mays
0.7
-
ATP 30°C, pH 8.5 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
gel filtration Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Zea mays
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme FK-I, FK-II Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
kernel
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Zea mays ADP + D-fructose 6-phosphate
-
?
CTP + D-fructose 18% of the activity with ATP Zea mays CDP + D-fructose 6-phosphate
-
?
GTP + D-fructose
-
Zea mays GDP + D-fructose 6-phosphate
-
?
UTP + D-fructose 14% of the activity with ATP Zea mays UDP + D-fructose 6-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5 broad with maximum at pH 8.5 Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.42
-
D-fructose FK 1, 30°C, pH 8.5 Zea mays
2.08
-
D-fructose FK 2, 30°C, pH 8.5 Zea mays