Application | Comment | Organism |
---|---|---|
medicine | potential target for antibiotics due to the fact, that the pathway is not found in mammals | Schizosaccharomyces pombe |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
ATP | pH 7.5, 30°C | Schizosaccharomyces pombe | |
0.52 | - |
L-homoserine | pH 7.5, 30°C | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | Schizosaccharomyces pombe | involved in the biosynthesis of threonine and isoleucine | ADP + O-phospho-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Schizosaccharomyces pombe |
Storage Stability | Organism |
---|---|
-80°C, 20 mM HEPES, pH 8.0, 10% glycerol, storage of purified enzyme | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | - |
Schizosaccharomyces pombe | ADP + O-phospho-L-homoserine | - |
? | |
ATP + L-homoserine | involved in the biosynthesis of threonine and isoleucine | Schizosaccharomyces pombe | ADP + O-phospho-L-homoserine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40.2 | - |
L-homoserine | pH 7.5, 30°C | Schizosaccharomyces pombe | |
40.8 | - |
ATP | pH 7.5, 30°C | Schizosaccharomyces pombe |