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Literature summary for 2.7.1.39 extracted from

  • Huo, X.; Viola, R.E.
    Functional group characterization of homoserine kinase from Escherichia coli (1996), Arch. Biochem. Biophys., 330, 373-379.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the combination of pTZ19u(ThrB) and the BL21(DE3) cell line is the best expression system with nearly 160 mg of enzyme produced in 4 liters of growth medium Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate
-
Escherichia coli
L-homoserine substrate inhibition Escherichia coli
pyridoxal 5'-phosphate
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-homoserine Escherichia coli key enzyme in the aspartate pathway of amino acid biosynthesis in E. coli ADP + O-phospho-L-homoserine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homoserine
-
Escherichia coli ADP + O-phospho-L-homoserine
-
?
ATP + L-homoserine key enzyme in the aspartate pathway of amino acid biosynthesis in E. coli Escherichia coli ADP + O-phospho-L-homoserine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.1
-
L-homoserine in 20% DMSO Escherichia coli
3
-
L-homoserine aqueous sulution, pH 8 Escherichia coli