BRENDA - Enzyme Database show
show all sequences of 2.7.1.39

Homoserine kinase of Escherichia coli: kinetic mechanism and inhibition by L-aspartate semialdehyde

Shames, S.L.; Wedler, F.C.; Arch. Biochem. Biophys. 235, 359-370 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5'-adenylylimidodiphosphate
-
Escherichia coli
L-alpha-aminobutyric acid
-
Escherichia coli
L-aspartate semialdehyde
mixed inhibition versus L-homoserine and ATP
Escherichia coli
L-homoserine
above 0.75 mM
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
L-homoserine
pH 7.8
Escherichia coli
0.58
-
L-aspartate semialdehyde
pH 7.8
Escherichia coli
3.8
-
4-hydroxynorvaline
pH 7.8
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Reaction
Reaction
Commentary
Organism
ATP + L-homoserine = ADP + O-phospho-L-homoserine
ordered random mechanism with ATP preferentially binding before L-homoserine
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-hydroxynorvaline
0.3% of the activity with L-homoserine
641463
Escherichia coli
ADP + 4-phospho-L-norvaline
-
-
-
?
ATP + L-aspartate 4-semialdehyde
10% of the activity with L-homoserine
641463
Escherichia coli
?
-
-
-
?
ATP + L-homoserine
-
641463
Escherichia coli
ADP + O-phospho-L-homoserine
-
641463
Escherichia coli
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
stable up to in absence of ligands
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.15
-
L-alpha-aminobutyric acid
-
Escherichia coli
0.9
-
5'-adenylylimidodiphosphate
pH 7.4
Escherichia coli
2
-
L-homoserine
pH 7.6
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5'-adenylylimidodiphosphate
-
Escherichia coli
L-alpha-aminobutyric acid
-
Escherichia coli
L-aspartate semialdehyde
mixed inhibition versus L-homoserine and ATP
Escherichia coli
L-homoserine
above 0.75 mM
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.15
-
L-alpha-aminobutyric acid
-
Escherichia coli
0.9
-
5'-adenylylimidodiphosphate
pH 7.4
Escherichia coli
2
-
L-homoserine
pH 7.6
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
L-homoserine
pH 7.8
Escherichia coli
0.58
-
L-aspartate semialdehyde
pH 7.8
Escherichia coli
3.8
-
4-hydroxynorvaline
pH 7.8
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-hydroxynorvaline
0.3% of the activity with L-homoserine
641463
Escherichia coli
ADP + 4-phospho-L-norvaline
-
-
-
?
ATP + L-aspartate 4-semialdehyde
10% of the activity with L-homoserine
641463
Escherichia coli
?
-
-
-
?
ATP + L-homoserine
-
641463
Escherichia coli
ADP + O-phospho-L-homoserine
-
641463
Escherichia coli
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
stable up to in absence of ligands
Escherichia coli
Other publictions for EC 2.7.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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722152
Kingsbury
Fungal homoserine kinase (thr1 ...
Candida albicans, Saccharomyces cerevisiae
Eukaryot. Cell
9
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2010
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2
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704525
Stockbridge
The intrinsic reactivity of AT ...
Methanocaldococcus jannaschii
J. Biol. Chem.
284
22747-22757
2009
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2
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706213
van Damme
Downy mildew resistance in Ara ...
Arabidopsis thaliana
Plant Cell
21
2179-2189
2009
-
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1
-
1
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5
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1
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1
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690392
Rinder
Regulation of aspartate-derive ...
Escherichia coli
Amino Acids
34
213-222
2008
-
1
1
-
1
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3
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694038
Kingsbury
Threonine biosynthetic genes a ...
Cryptococcus neoformans
Microbiology
154
2767-2775
2008
-
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1
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2
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663091
Lee
Methionine and threonine synth ...
Arabidopsis thaliana
Plant J.
41
685-696
2005
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1
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2
2
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1
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4
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673979
Paik
Identification of virulence de ...
Streptococcus sanguinis, Streptococcus sanguinis SK36
Infect. Immun.
73
6064-6074
2005
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661325
Bareich
Small molecule functional disc ...
Schizosaccharomyces pombe
Bioorg. Med. Chem.
12
807-815
2004
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1
1
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662779
Fernandez
Role of homoserine and threoni ...
Streptomyces sp., Streptomyces sp. NRRL 5331
Microbiology
150
1467-1474
2004
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641475
Krishna
Structural basis for the catal ...
Methanocaldococcus jannaschii
Biochemistry
40
10810-10818
2001
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1
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641474
Zhou
Structure and mechanism of hom ...
Methanocaldococcus jannaschii
Structure Fold. Des.
8
1247-1257
2000
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641472
Lee
Identification of the gene enc ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
372
135-142
1999
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1
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641476
Patte
ThrH, a homoserine kinase isoz ...
Pseudomonas aeruginosa
Microbiology
145
845-853
1999
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641471
Huo
Substrate specificity and iden ...
Escherichia coli
Biochemistry
35
16180-16185
1996
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6
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16
26
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1
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13
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1
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8
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16
8
26
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13
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1
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641473
Huo
Functional group characterizat ...
Escherichia coli
Arch. Biochem. Biophys.
330
373-379
1996
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641467
Riesmeier
-
Purification to homogeneity an ...
Triticum aestivum
Phytochemistry
32
581-584
1993
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641468
Ramos
Inhibition by different amino ...
Saccharomyces cerevisiae
FEBS Lett.
278
123-126
1991
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2
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641469
Mannhaupt
Yeast homoserine kinase. Chara ...
Saccharomyces cerevisiae
Eur. J. Biochem.
191
115-122
1990
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1
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2
1
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2
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1
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641464
Finkelnburg
-
Homoserine kinase from phototr ...
Rhodospirillum rubrum, Rhodospirillum rubrum S1
FEMS Microbiol. Lett.
48(1-2)
93-96
1987
1
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9
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641463
Shames
Homoserine kinase of Escherich ...
Escherichia coli
Arch. Biochem. Biophys.
235
359-370
1984
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641462
Baum
-
Feedback inhibition of homoser ...
Raphanus sativus
Phytochemistry
22
2409-2412
1983
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641461
Thoen
-
Biosynthesis of threonine from ...
Pisum sativum
Plant Sci. Lett.
13
103-112
1978
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641460
Aarnes
-
Homoserine kinase from barley ...
Hordeum vulgare
Plant Sci. Lett.
7
187-194
1976
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641470
Burr
Homoserine kinase from Escheri ...
Escherichia coli
Eur. J. Biochem.
62
519-526
1976
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9
2
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641465
Theze
Homoserine kinase from Escheri ...
Escherichia coli
J. Bacteriol.
118
577-581
1974
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641466
Miyajima
Regulation of aspartate family ...
Brevibacterium flavum
J. Biochem.
71
219-226
1972
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