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Literature summary for 2.7.1.36 extracted from

  • Kudoh, T.; Park, C.S.; Lefurgy, S.T.; Sun, M.; Michels, T.; Leyh, T.S.; Silverman, R.B.
    Mevalonate analogues as substrates of enzymes in the isoprenoid biosynthetic pathway of Streptococcus pneumoniae (2009), Bioorg. Med. Chem., 18, 1124-1134.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as dual-tagged His6-GST fusion protein Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
diphosphomevalonate strong feedback inhibitor of the enzyme Streptococcus pneumoniae
additional information synthesis of a series of C3-substituted mevalonate analogues to probe the steric and electronic requirements of the mevalonate kinase active site for development of specific mechanism-based inhibitors, overview Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Streptococcus pneumoniae
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q8DR51
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant dual-tagged His6-GST enzyme from Escherichia coli by affinity chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
 Streptococcus pneumoniae ADP + (R)-5-phosphomevalonate
-
 ?
additional information the recombinant enzyme phosphorylates racemic vinyl, ethyl, n-propyl, n-butyl, i-butyl, 2-propenyl, allyl, ethynyl, and 1-propynyl mevalonate lactone analogues, that are diphosphorylated by phosphomevalonate kinase, EC 2.7.4.2, in a second step, overview Streptococcus pneumoniae ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Streptococcus pneumoniae

General Information

General Information Comment Organism
metabolism the enzyme is regulating the mevalonate pathway in Streptococcus pneumoniae, it is strongly feedback-inhibited by diphosphomevalonate, the penultimate compound in the pathway, overview Streptococcus pneumoniae