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Literature summary for 2.7.1.36 extracted from

  • Andreassi, J.L.; Bilder, P.W.; Vetting, M.W.; Roderick, S.L.; Leyh, T.S.
    Crystal structure of the Streptococcus pneumoniae mevalonate kinase in complex with diphosphomevalonate (2007), Protein Sci., 16, 983-989.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with diphosphomevalonate and Mg2+, diffraction to 2.5 A resolution. Diphosphomevalonate functions as a partial bisubstrate analogue and elicits a ternary-complex like form of the enzyme Streptococcus pneumoniae
purified enzyme in complex with 5-diphosphomevalonate and Mg2+ bound to the active cleft, sitting drop vapour diffusion method, 0.002 ml of 6,0 mg/ml protein in 50 mM HEPES, pH 7.5, 50 mM KCl, 1 mM DTT, 10 mM adenosine 5'-(beta,gamma-imino)triphosphate, 12 mM MgCl2, and 0.5 mM diphosphomevalonate is mixed with 0.002 ml of precipitatnt solution containing 40% v/v PEG 400, and 200 mM sodium formate, at room temperature, X-ray diffraction structure determination and anaylsis at 2.5 A resolution, single-wavelength anomalous diffraction method Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
5-diphosphomevalonate allosteric and potent inhibition, the molecule functions as a partial bisubstrate analogue that elicits a ternary-complex-like form of the enzyme, binding structure, overview Streptococcus pneumoniae
diphosphomevalonate functions as a partial bisubstrate analogue and elicits a ternary-complex like form of the enzyme Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ binding structure, overview Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Streptococcus pneumoniae the enzyme catalyzes a step in the isoprenoid biosynthetic pathway via the essential intermediate 5-diphosphomevalonate ADP + (R)-5-phosphomevalonate
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Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate the enzyme catalyzes a step in the isoprenoid biosynthetic pathway via the essential intermediate 5-diphosphomevalonate Streptococcus pneumoniae ADP + (R)-5-phosphomevalonate
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ATP + (R)-mevalonate substrate binding structure, overview Streptococcus pneumoniae ADP + (R)-5-phosphomevalonate
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Cofactor

Cofactor Comment Organism Structure
ATP
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Streptococcus pneumoniae