Application | Comment | Organism |
---|---|---|
medicine | potential target for drugs | Enterococcus faecalis |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Enterococcus faecalis | |
additional information | no inhibition with AMP | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
GTP | pH 10, 37°C | Enterococcus faecalis | |
0.33 | - |
(R)-mevalonate | pH 10, 37°C | Enterococcus faecalis | |
0.8 | - |
CTP | pH 10, 37°C | Enterococcus faecalis | |
1.1 | - |
ATP | pH 10, 37°C | Enterococcus faecalis | |
1.4 | - |
ITP | pH 10, 37°C | Enterococcus faecalis | |
1.9 | - |
TTP | pH 10, 37°C | Enterococcus faecalis | |
2.8 | - |
UTP | pH 10, 37°C | Enterococcus faecalis | |
3.3 | - |
Mg2+ | pH 10, 37°C | Enterococcus faecalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Enterococcus faecalis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace for Mg2+ | Enterococcus faecalis | |
Mg2+ | required | Enterococcus faecalis | |
Mn2+ | can replace for Mg2+ | Enterococcus faecalis | |
Zn2+ | can replace for Mg2+ | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32200 | - |
sedimentation velocity | Enterococcus faecalis |
33840 | - |
calculated from the deduced amino acid sequence | Enterococcus faecalis |
71100 | - |
sedimentation velocity, 10% of total enzyme | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Enterococcus faecalis | involved in isopentenyl diphosphate synthesis | ADP + (R)-5-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate | sequential mechanism | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
purified enzyme, pH 10, 37°C | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Enterococcus faecalis | ADP + (R)-5-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | involved in isopentenyl diphosphate synthesis | Enterococcus faecalis | ADP + (R)-5-phosphomevalonate | - |
? | |
CTP + (R)-mevalonate | - |
Enterococcus faecalis | CDP + (R)-5-phosphomevalonate | - |
? | |
GTP + (R)-mevalonate | - |
Enterococcus faecalis | GDP + (R)-5-phosphomevalonate | - |
? | |
ITP + (R)-mevalonate | - |
Enterococcus faecalis | IDP + (R)-5-phosphomevalonate | - |
? | |
TTP + (R)-mevalonate | - |
Enterococcus faecalis | TDP + (R)-5-phosphomevalonate | - |
? | |
UTP + (R)-mevalonate | - |
Enterococcus faecalis | UDP + (R)-5-phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32200, sedimentation velocity, 10% of total enzyme | Enterococcus faecalis |
monomer | 1 * 32200, sedimentation velocity, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Enterococcus faecalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | - |
Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Enterococcus faecalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | - |
Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
ADP | pH 10, 37°C | Enterococcus faecalis |