Activating Compound | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 10 mM, 20% activation | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | very weak inhibition | Phaseolus vulgaris | |
CuSO4 | 10 mM, almost complete inhibition | Phaseolus vulgaris | |
farnesyl diphosphate | 0.3 mM, approx. 75% inhibition | Phaseolus vulgaris | |
geranyl diphosphate | 0.3 mM, approx. 10% inhibition | Phaseolus vulgaris | |
geranylgeranyl diphosphate | 0.3 mM, approx. 90% inhibition | Phaseolus vulgaris | |
mevalonate | at high concentrations | Phaseolus vulgaris | |
mevalonate 5-phosphate | 0.3 mM, approx. 45% inhibition | Phaseolus vulgaris | |
Mn2+ | inhibition at high concentrations | Phaseolus vulgaris | |
p-chloromercuribenzoate | 1 mM, almost complete inhibition | Phaseolus vulgaris | |
phytyl diphosphate | 0.3 mM, almost complete inhibition | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0426 | - |
RS-mevalonate | pH 7.0, 30°C, enzyme from etiolated cotyledons | Phaseolus vulgaris | |
0.046 | - |
RS-mevalonate | pH 7.0, 30°C, leaf enzyme | Phaseolus vulgaris | |
1.54 | - |
ATP | pH 7.0, 30°C, enzyme from etiolated cotyledons | Phaseolus vulgaris | |
1.75 | - |
ATP | pH 7.0, 30°C, enzyme fom green leaves | Phaseolus vulgaris | |
2 | - |
ATP | pH 7.0, 30°C | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 9 mM | Phaseolus vulgaris | |
Mn2+ | most active at low concentrations | Phaseolus vulgaris | |
Mn2+ | maximal activation at 2 mM | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | 104000 | gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme from cotyledons and leaves, ammonium sulfate, Sephadex G-200, DEAE-Sephadex | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | etiolated | Phaseolus vulgaris | - |
leaf | - |
Phaseolus vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + mevalonate | - |
Phaseolus vulgaris | ? | - |
? | |
ATP + mevalonate | - |
Phaseolus vulgaris | ADP + phosphomevalonate | - |
? | |
CTP + mevalonate | weak activity | Phaseolus vulgaris | CDP + phosphomevalonate | - |
? | |
GTP + mevalonate | weak activity | Phaseolus vulgaris | GDP + phosphomevalonate | - |
? | |
ITP + mevalonate | - |
Phaseolus vulgaris | IDP + phosphomevalonate | - |
? | |
UTP + mevalonate | - |
Phaseolus vulgaris | UDP + phosphomevalonate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Phaseolus vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | approx. 30% of maximal activity at pH 5.5, approx. 60% of maximal activity at pH 9 | Phaseolus vulgaris |