Literature summary for 2.7.1.35 extracted from

Kim, J.H.; Kim, J.; Kim, H.J.; Sathiyanarayanan, G.; Bhatia, S.K.; Song, H.S.; Choi, Y.K.; Kim, Y.G.; Park, K.; Yang, Y.H.
Biotransformation of pyridoxal 5-phosphate from pyridoxal by pyridoxal kinase (pdxY) to support cadaverine production in Escherichia coli (2017), Enzyme Microb. Technol., 104, 9-15 .

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Application

Application	Comment	Organism
synthesis	production of cadaverine (1,5-diaminopentane) can be done by fermentation or direct bioconversion and plays an important role as a building block of polyamides. Lysine decarboxylase (CadA) transforms L-lysine to cadaverine and pyridoxal 5'-phosphate (PLP) can increase the conversion rate and yield as a cofactor. Biotransformation of cadaverine using whole Escherichia coli cells that overexpress lysine decarboxylase has many merits, such as the rapid conversion of L-lysine to cadaverine, possible application of high concentration reactions up to the molar level, production of less byproduct, and potential reuse of the enzyme by immobilization. But the supply of PLP, an essential cofactor of lysine decarboxylase, is the major bottleneck in this system. Among various PLP systems examined, pyridoxal kinase (PdxY) shows the highest conversion of PL to PLP, resulting in more than 60 % conversion of L-lysine to cadaverine with lysine decarboxylase. Method evaluation and optimization. Interconversion of interconvertion of pyridoxal and pyridoxamine has to be controlled	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
gene pdxY, cloned into pET23a::pdxY (p23YH) or pET23a:: pdxK (p23KH) as a one-vector system in Escherichia coli strain DH5alpha and transformed into Escherichia coli strain BL21(DE3)	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Escherichia coli	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + pyridoxal	Escherichia coli	-	ADP + pyridoxal 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P77150	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyridoxal	-	Escherichia coli	ADP + pyridoxal 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
pdxY	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	biotransformation of pyridoxal to pyridoxal 5'-phosphate (PLP) by pyridoxal kinase (pdxY) supports cadaverine production in Escherichia coli. PLP is an essential cofactor of lysine decarboxylase and the major bottleneck in the cadaverine biosynthesis pathway	Escherichia coli

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Release 2023.1 (January 2023)