Cloned (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
native protein and in complex with its substrates to 1.4?1.85 A resolution. The protein shows a typical ribokinase fold with a central large beta-sheet consisting of nine strands, flanked by three and five structurally conserved alpha-helices | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
C110A | residue C110 is mandatory for pyridoxal, but not for pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine turnover | Staphylococcus aureus |
C214A | mutant does not turn over pyridoxal, pyridoxine, or 4-amino-5-hydroxymethyl-2-methylpyrimidine. Residue C214 acts as the catalytic base in the phosphorylation reaction | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rugulactone | selectively modifies the enzyme not at the active site cysteine, but on a remote cysteine residue | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
pyridoxal | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.26 | - |
pyridoxal | mutant C214D, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
1.51 | - |
pyridoxine | mutant C110A, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
1.85 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | mutant C110A, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
1.99 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
2.07 | - |
pyridoxine | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A0H3JTP0 | - |
- |
Staphylococcus aureus ATCC 700699 | A0A0H3JTP0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | reaction of EC 2.7.1.49 | Staphylococcus aureus | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | reaction of EC 2.7.1.49 | Staphylococcus aureus ATCC 700699 | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
ATP + pyridoxal | - |
Staphylococcus aureus | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Staphylococcus aureus ATCC 700699 | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxine | - |
Staphylococcus aureus | ADP + pyridoxine 5'-phosphate | - |
? | |
ATP + pyridoxine | - |
Staphylococcus aureus ATCC 700699 | ADP + pyridoxine 5'-phosphate | - |
? | |
additional information | the reactive Cys110 residue in the lid region forms a hemithioactetal intermediate with the 4'-aldehyde of pyridoxal. This hemithioacetal, in concert with the catalytic Cys214, increases the nucleophilicity of the pyridoxal 5'-OH group for the inline displacement reaction with the gamma-phosphate of ATP | Staphylococcus aureus | ? | - |
? | |
additional information | the reactive Cys110 residue in the lid region forms a hemithioactetal intermediate with the 4'-aldehyde of pyridoxal. This hemithioacetal, in concert with the catalytic Cys214, increases the nucleophilicity of the pyridoxal 5'-OH group for the inline displacement reaction with the gamma-phosphate of ATP | Staphylococcus aureus ATCC 700699 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAV0580 | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
pyridoxine | mutant C110A, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.016 | - |
pyridoxine | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.044 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | mutant C110A, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.045 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.165 | - |
pyridoxal | wild-type, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus | |
0.328 | - |
pyridoxal | mutant C214D, pH not specified in the publication, temperature not specified in the publication | Staphylococcus aureus |