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Literature summary for 2.7.1.35 extracted from

  • Jones, D.C.; Alphey, M.S.; Wyllie, S.; Fairlamb, A.H.
    Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome (2012), Mol. Microbiol., 86, 51-64.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
4-deoxypyridoxine
-
 Trypanosoma brucei
Ca2+ low substrate inhibition Trypanosoma brucei
ginkgotoxin i.e.4'-O-methylpyridoxine Trypanosoma brucei
Mg2+ low substrate inhibition Trypanosoma brucei
roscovitine
-
 Trypanosoma brucei
Zn2+ ZnCl2 shows pronounced high substrate inhibition Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00165
-
 pyridoxal recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.038
-
 pyridoxal recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.0466
-
 ginkgotoxin recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.048
-
 pyridoxine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.0594
-
 pyridoxamine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Trypanosoma brucei
Cu2+
-
 Trypanosoma brucei
Fe2+
-
 Trypanosoma brucei
Mg2+ best activator, optimum activity at 0.25 mM Mn2+ Trypanosoma brucei
Mn2+ optimum activity at 0.05 mM Mn2+ Trypanosoma brucei
additional information ZnCl2 and Ca2+ do not activate the enzyme Trypanosoma brucei
Ni2+
-
 Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Superdex 75 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ginkgotoxin ginkgotoxin is an effective pseudosubstrate Trypanosoma brucei ?
-
 ?
ATP + pyridoxal
-
 Trypanosoma brucei ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Trypanosoma brucei ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Trypanosoma brucei ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
PdxK
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106
-
 ginkgotoxin recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
122
-
 pyridoxine recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
127
-
 pyridoxamine recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
131
-
 pyridoxal recombinant enzyme, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0398
-
 Zn2+ recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
1.54
-
 Mg2+ recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei

General Information

General Information Comment Organism
physiological function the enzyme is essential for growth of Trypanosoma brucei in vitro and for infectivity in mice Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000214
-
 pyridoxamine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.000228
-
 ginkgotoxin recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.000254
-
 pyridoxine recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei
0.0009
-
 pyridoxal recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C Trypanosoma brucei