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Literature summary for 2.7.1.35 extracted from

  • Cao, P.; Gong, Y.; Tang, L.; Leung, Y.C.; Jiang, T.
    Crystal structure of human pyridoxal kinase (2006), J. Struct. Biol., 154, 327-332.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) plysS Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 0.1 mM Tris-HCl pH 8.0, 1.8 M NH4Ac, and 3% glycol Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O00764
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and HiLoad 26/60 Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
Homo sapiens ADP + pyridoxal 5'-phosphate
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
HPLK
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens