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Literature summary for 2.7.1.35 extracted from

  • di Salvo, M.L.; Hunt, S.; Schirch, V.
    Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases (2004), Protein Expr. Purif., 36, 300-306.
    View publication on PubMed
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HMS174(DE3) Homo sapiens
kinase 1 and 2 expressed in Escherichia coli HMS174(DE3), kinase 2 additionally expressed as His-tag fusion protein Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 pyridoxamine kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.015
-
 pyridoxine with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.02
-
 pyridoxine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.025
-
 pyridoxine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.03
-
 pyridoxamine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.03
-
 pyridoxal with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.035
-
 pyridoxamine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.07
-
 ATP kinase 1 with pyridoxal as substrate, pH 7.3, 37°C Escherichia coli
0.07
-
 ATP ATP in form of ZnATP2- Escherichia coli
0.1
-
 pyridoxal kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.19
-
 pyridoxal kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.35
-
 pyridoxal with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.42
-
 ATP with pyridoxal as substrate, pH 7.3, 37°C Homo sapiens
0.42
-
 ATP ATP in form of MgATP Homo sapiens
0.6
-
 ATP kinase 1 with pyridoxal as substrate, pH 7.3, 37°C Escherichia coli
0.6
-
 ATP ATP in form of MgATP2- Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in complex with ATP most likely preferred substrate in vivo Escherichia coli
Mg2+ in complex with ATP most likely preferred substrate in vivo Homo sapiens
Zn2+ in complex with ATP preferred substrate in vitro Escherichia coli
Zn2+ in complex with ATP preferred substrate in vitro Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyridoxal Escherichia coli generates the active form of vitamin B6, which serves as cofactor for many enzymes ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Homo sapiens generates the active form of vitamin B6, which serves as cofactor for many enzymes ADP + pyridoxal 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 kinase 1
-
Homo sapiens O00764
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.052
-
 purified kinase 2 enzyme, pH 6.1, 37°C Escherichia coli
2.5
-
 purified enzyme, pH 6.1, 37°C Homo sapiens
2.7
-
 purified kinase 1 enzyme, pH 6.1, 37°C Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, 20 mM phosphate buffer, pH 7.3, stable for at least some weeks Escherichia coli
-80°C, 20 mM phosphate buffer, pH 7.3, stable for at least some weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Escherichia coli ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal generates the active form of vitamin B6, which serves as cofactor for many enzymes Escherichia coli ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal generates the active form of vitamin B6, which serves as cofactor for many enzymes Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Escherichia coli ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Homo sapiens ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Escherichia coli ADP + pyridoxine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Homo sapiens ADP + pyridoxine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ePL kinase two different forms encoded by genes PdxK and PdxY yielding kinase 1 and kinase 2, low activity of kinase 2 suggests that this kinase originally has another substrate but can also utilize pyridoxal Escherichia coli
hPL kinase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 pyridoxine with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.3
-
 pyridoxine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.3
-
 pyridoxine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.3
-
 pyridoxamine with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.4
-
 pyridoxamine kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.5
-
 pyridoxal with ZnATP2- as substrate, pH 7.3, 37°C Homo sapiens
0.7
-
 pyridoxamine kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli
0.8
-
 pyridoxal with MgATP2- as substrate, pH 7.3, 37°C Homo sapiens
2
-
 pyridoxal kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C Escherichia coli
2.3
-
 pyridoxal kinase 1 with MgATP2- as substrate, pH 7.3, 37°C Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli kinase 1, calculated from the deduced amino acid sequence
-
 5.14
Homo sapiens calculated from the deduced amino acid sequence
-
 5.75
Escherichia coli kinase 2, calculated from the deduced amino acid sequence
-
 6.05