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Literature summary for 2.7.1.33 extracted from

  • Yao, J.; Subramanian, C.; Rock, C.O.; Jackowski, S.
    Human pantothenate kinase 4 is a pseudo-pantothenate kinase (2019), Protein Sci., 28, 1031-1047 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.33

Cloned(Commentary)

Cloned (Comment) Organism
gene PANK3, sequence comparisons and phylogenetic analysis Homo sapiens
gene PANK4, sequence comparisons and phylogenetic analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
E138V site-directed mutagenesis, inactive mutant Homo sapiens
E138V naturally occuring mutation in isozyme PanK4, inactive mutant Homo sapiens
additional information human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity Homo sapiens
R207W site-directed mutagenesis, inactive mutant Homo sapiens
R207W naturally occuring mutation in isozyme PanK4, inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens
0.0031
-
 ATP pH 7.5, 37°C, wild-type enzyme Homo sapiens
0.017
-
 (R)-pantothenate pH 7.5, 37°C, wild-type enzyme Homo sapiens
0.025
-
 ATP pH 7.5, 37°C, mutant PanK3 R107W Homo sapiens
0.833
-
 (R)-pantothenate pH 7.5, 37°C, mutant PanK3 R107W Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantothenate Homo sapiens
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9H999
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Homo sapiens ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

Synonyms Comment Organism
HsPANK3
-
 Homo sapiens
HsPANK4
-
 Homo sapiens
PanK3
-
 Homo sapiens
PanK4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution the human genome encodes three well-characterized and nearly identical pantothenate kinases (PANK1-3) plus a putative bifunctional protein (PANK4) with a predicted amino-terminal pantothenate kinase domain fused to a carboxy-terminal phosphatase domain. Structural and phylogenetic analyses show that all active, characterized PANKs contain the key catalytic residues Glu138 and Arg207 (HsPANK3 numbering). All amniote PANK4s, including human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Evolutionary history of PANK, phylogenetic analysis, overview Homo sapiens
evolution the human genome encodes three well-characterized and nearly identical pantothenate kinases (PANK1-3) plus a putative bifunctional protein (PANK4) with a predicted N-terminal pantothenate kinase domain fused to a C-terminal phosphatase domain. Structural and phylogenetic analyses show that all active, characterized PANKs contain the key catalytic residues Glu138 and Arg207 (HsPANK3 numbering). All amniote PANK4s, including human PANK4, encode Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Evolutionary history of PANK, overview Homo sapiens
malfunction human PANK3 is inactivated by mutations Glu138Val and Arg207Trp Homo sapiens
malfunction human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi. Pank4 encodes Glu138Val and Arg207Trp substitutions which are predicted to inactivate kinase activity Homo sapiens
additional information sequence comparison of isozyme PanK3 and PanK4 Homo sapiens
physiological function pantothenate kinase generates 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) biosynthesis. Critical roles of Glu138 and Arg207 in HsPANK3 Homo sapiens
physiological function pantothenate kinase generates 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) biosynthesis. Isozyme PanK4 is a putative bifunctional protein with a predicted amino-terminal pantothenate kinase domain fused to a carboxy-terminal phosphatase domain. HsPANK4 has reduced kinase activity prior to the catalytic residue substitutions in amniotes. Human PANK4 is a pseudo-pantothenate kinase, a catalytically deficient variant of the catalytically active PANK4 found in plants and fungi Homo sapiens