Cloned (Comment) | Organism |
---|---|
gene CAB1, recombinant overexpression of PanK under a strong constitutive promoter (truncated HXT7 promoter) in Saccharomyces cerevisiae strain CENF09 resulting in strain CENFP01 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | improvement of acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass. PanK overexpression or PDH bypass introduction alone only lead to a 2.0fold or 6.74fold increase in naringenin titer, but the combination of both (strain CENFPAA01) results in 24.4fold increase as compared to the control (strain CENF09) in the presence of 0.5 mM substrate p-coumaric acid. The supplement of PanK substrate pantothenate results in another 19% increase in naringenin production | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Saccharomyces cerevisiae | - |
ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | Saccharomyces cerevisiae BY4742 | - |
ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | Saccharomyces cerevisiae ATCC 204508 | - |
ADP + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04430 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | Q04430 | - |
- |
Saccharomyces cerevisiae BY4742 | Q04430 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Saccharomyces cerevisiae | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Saccharomyces cerevisiae BY4742 | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Saccharomyces cerevisiae ATCC 204508 | ADP + (R)-4'-phosphopantothenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cab1 | - |
Saccharomyces cerevisiae |
PanK | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | PanK is the rate-limiting enzyme for CoA synthesis in Saccharomyces cerevisiae, acetyl-CoA metabolism and associated naringenin synthesis pathway overview | Saccharomyces cerevisiae |
physiological function | pantothenate, the substrate for PanK and precursor for CoA, is directly related with acetyl-CoA biosynthesis in Saccharomyces cerevisiae. PanK is the rate-limiting step for CoA synthesis and pantothenate supplement helps to increase the CoA/acetyl-CoA level in mammalian and Escherichia coli cells, as well as in Saccharomyces cerevisiae cells | Saccharomyces cerevisiae |