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Literature summary for 2.7.1.33 extracted from

  • Hughes, S.J.; Antoshchenko, T.; Kim, K.P.; Smil, D.; Park, H.W.
    Structural characterization of a new N-substituted pantothenamide bound to pantothenate kinases from Klebsiella pneumoniae and Staphylococcus aureus (2014), Proteins, 82, 1542-1548.
    View publication on PubMed
Search for more literature for 2.7.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with N-[2-(1,3-benzodioxol-5-yl)ethyl] pantothenamide, hanging drop vapor diffusion method, using 1.4 M sodium citrate, 0.1 M HEPES pH 7.5 and 5% (v/v) ethylene glycol Staphylococcus aureus
in complex with N-[2-(1,3-benzodioxol-5-yl)ethyl] pantothenamide, hanging drop vapor diffusion method, using 2 M sodium formate and 0.1 M Tris-HCl pH 8.5 Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantothenate Staphylococcus aureus
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate Klebsiella pneumoniae
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate Staphylococcus aureus MSSA476
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate Klebsiella pneumoniae 342
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae B5XYG3
-
-
Klebsiella pneumoniae 342 B5XYG3
-
-
Staphylococcus aureus Q6G7I0
-
-
Staphylococcus aureus MSSA476 Q6G7I0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Staphylococcus aureus
Ni-NTA resin column chromatography and Superdex 200 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Klebsiella pneumoniae ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Staphylococcus aureus MSSA476 ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Klebsiella pneumoniae 342 ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

Synonyms Comment Organism
PanK
-
 Staphylococcus aureus
PanK
-
 Klebsiella pneumoniae