Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.33 extracted from

  • Shimizu, S.; Kubo, K.; Tani, Y.; Ogata, K.
    Purification and properties of pantothenate kinase from brevibacterium ammoniagenes IFO 12071 (1973), Agric. Biol. Chem., 37, 2863-2870.
No PubMed abstract available
Search for more literature for 2.7.1.33

General Stability

General Stability Organism
ATP and/or sucrose do not stabilize Corynebacterium ammoniagenes

Inhibitors

Inhibitors Comment Organism Structure
ADP not Corynebacterium ammoniagenes
AlCl3 strong Corynebacterium ammoniagenes
Ba2+
-
 Corynebacterium ammoniagenes
CoA
-
 Corynebacterium ammoniagenes
Cu2+ strong Corynebacterium ammoniagenes
D-pantothenate substrate inhibition, above 0.5 mM Corynebacterium ammoniagenes
dephospho-CoA
-
 Corynebacterium ammoniagenes
EDTA
-
 Corynebacterium ammoniagenes
Fe2+ strong Corynebacterium ammoniagenes
HgCl2 strong Corynebacterium ammoniagenes
additional information no inhibition by 2,2'-dipyridyl, Ca2+, Cd2+, 3'-AMP, GTP, GDP, ITP, UTP; not inhibitory: 3',5'-ADP Corynebacterium ammoniagenes
Na2HAsO4
-
 Corynebacterium ammoniagenes
NH4+
-
 Corynebacterium ammoniagenes
pantetheine
-
 Corynebacterium ammoniagenes
pantetheine 4'-phosphate weak Corynebacterium ammoniagenes
pantothenic acid 4'-phosphate
-
 Corynebacterium ammoniagenes
pantothenoylcysteine 4'-phosphate weak Corynebacterium ammoniagenes
pantothenyl alcohol
-
 Corynebacterium ammoniagenes
Pb2+
-
 Corynebacterium ammoniagenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 ATP pH 6.5, 37°C Corynebacterium ammoniagenes
0.067
-
 pantothenate pH 6.5, 37°C Corynebacterium ammoniagenes
1
-
 ATP pH 6.5, 37°C Corynebacterium ammoniagenes
1
-
 ATP ATP in form of MgATP2- Corynebacterium ammoniagenes

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation, can replace Mg2+ Corynebacterium ammoniagenes
Mg2+ Km-value: 1 mM Corynebacterium ammoniagenes
Mg2+ requirement, active substrate: MgATP-complex Corynebacterium ammoniagenes
Mn2+ activation, can replace Mg2+ Corynebacterium ammoniagenes
additional information no activation by Ca2+, Cd2+, Ba2+, Pb2+, Fe2+ or Cu2+ Corynebacterium ammoniagenes
Ni2+ activation, can replace Mg2+ with about 50% efficiency Corynebacterium ammoniagenes
Zn2+ activation, can replace Mg2+ with about 50% efficiency Corynebacterium ammoniagenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Corynebacterium ammoniagenes

Organism

Organism UniProt Comment Textmining
Corynebacterium ammoniagenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium ammoniagenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.034
-
 pH 6.5, 37°C Corynebacterium ammoniagenes

Storage Stability

Storage Stability Organism
4°C, 2 mg protein/ml in 0.01 M phosphate buffer, pH 7, inactivation within 4 days Corynebacterium ammoniagenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Corynebacterium ammoniagenes ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + D-pantothenate
-
 Corynebacterium ammoniagenes ADP + D-4'-phosphopantothenate
-
 ?
GTP + pantothenate phosphorylation at 28% the rate of ATP Corynebacterium ammoniagenes GDP + D-4'-phosphopantothenate
-
 ?
ITP + pantothenate phosphorylation at 46% the rate of ATP Corynebacterium ammoniagenes IDP + D-4'-phosphopantothenate
-
 ?
pantetheine + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopantetheine + ADP
-
 ?
panthenoylcysteine + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopanthenoylcysteine + ADP
-
 ?
pantothenyl alcohol + ATP
-
 Corynebacterium ammoniagenes D-4'-phosphopantothenyl alcohol + ADP
-
 ?
UTP + pantothenate phosphorylation at 18% the rate of ATP Corynebacterium ammoniagenes UDP + D-4'-phosphopantothenate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Corynebacterium ammoniagenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 10% to 20% loss of activity within 10 min at pH 7.5 to 8, 10 min stable at pH 7, t1/2: 10 min at pH 6.5 Corynebacterium ammoniagenes
37
-
 10 min, 70% loss of activity Corynebacterium ammoniagenes
43
-
 10 min, inactivation Corynebacterium ammoniagenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Corynebacterium ammoniagenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 t1/2: 10 min at 30°C Corynebacterium ammoniagenes
7
-
 10 min stable at 30°C Corynebacterium ammoniagenes
7.5
-
 90% of activity retained after 10 min at 30°C Corynebacterium ammoniagenes
8
-
 80% of activity retained after 10 min at 30°C Corynebacterium ammoniagenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.033
-
 CoA reduced form, pH 6.5, 37°C Corynebacterium ammoniagenes
0.037
-
 pantetheine 4'-phosphate pH 6.5, 37°C Corynebacterium ammoniagenes
0.067
-
 CoA oxidized form, pH 6.5, 37°C Corynebacterium ammoniagenes
0.125
-
 pantothenic acid 4'-phosphate pH 6.5, 37°C Corynebacterium ammoniagenes