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Literature summary for 2.7.1.33 extracted from
- Coenzyme A biosynthesis in plants: partial purification and characterization of pantothenate kinase from spinach (1993), Arch. Biochem. Biophys., 301, 424-430.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Acyl carrier protein | PAK II: inhibition, PAK I: stimulation between 0.015-0.035 mM | Spinacia oleracea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | not | Brassica napus | |
| acetyl-CoA | not | Spinacia oleracea | |
| Acyl carrier protein | - |
Brassica napus | |
| Acyl carrier protein | PAK II: inhibition, PAK I: stimulation between 0.015-0.035 mM | Spinacia oleracea | |
| CoA | not | Brassica napus | |
| CoA | not | Spinacia oleracea | |
| malonyl-CoA | - |
Brassica napus | |
| malonyl-CoA | 0.075 mM, complete inhibition of PAK I | Spinacia oleracea | |
| additional information | not inhibitory: 3',5'-ADP; not inhibitory: CoASH, acetyl-CoA | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
pantothenate | isoform 2, 37°C, pH 8.0 | Spinacia oleracea | |
| 1.3 | - |
pantothenate | isoform 1, 37°C, pH 8.0 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement, active substrate: MgATP-complex | Spinacia oleracea | |
| Mg2+ | requirement, active substrate: MgATP-complex | Brassica napus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-pantothenate | Spinacia oleracea | - |
ADP + D-4'-phosphopantothenate | - |
? | |
| ATP + D-pantothenate | Brassica napus | - |
ADP + D-4'-phosphopantothenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
- |
- |
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, presumably 2 isoforms | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
| seedling | - |
Spinacia oleracea | - |
| seedling | - |
Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.152 | - |
isoform 1, 37°C, pH 8.0 | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, t1/2: 3 days | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-pantothenate | - |
Spinacia oleracea | ADP + D-4'-phosphopantothenate | - |
? | |
| ATP + D-pantothenate | - |
Brassica napus | ADP + D-4'-phosphopantothenate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
broad | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Spinacia oleracea |
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