Literature summary for 2.7.1.32 extracted from

Torretta, A.; Lopez-Cara, L.; Parisini, E.
Crystal structure of the apo and the adp-bound form of choline kinase from Plasmodium falciparum (2020), Crystals, 10, 1-13 .

No PubMed abstract available

Application

Application	Comment	Organism
drug development	targeting the parasite lipid metabolism and choline kinase for pharmacological action constitutes an effective way of dealing with the spreading of the disease and with conventional therapy-resistant strains	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Star	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinat His6-tagged choline kinase in apoform and in ADP-bound form, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein solution with 0.001 ml of reservoir solution, and equilibration against 0.5 ml reservoir solution, containing 16% v/v PEG 8000, 0.2 M NaCl, 0.1 M HEPES, pH 7.5, 2 mM Tris(2-carboxyethyl)phosphine (TCEP), and 4 mM MgCl2 for the apoform crystals, and the same composition with 2 mM ADP for the complex crystals, at room temperature, X-ray diffraction structrue determination and analysis at 2.0-2.2 A resolution, molecular replacement and model building using the PDB ID 3FI8 structure as starting model	Plasmodium falciparum

Crystallization (Comment)

Organism

purified recombinat His6-tagged choline kinase in apoform and in ADP-bound form, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein solution with 0.001 ml of reservoir solution, and equilibration against 0.5 ml reservoir solution, containing 16% v/v PEG 8000, 0.2 M NaCl, 0.1 M HEPES, pH 7.5, 2 mM Tris(2-carboxyethyl)phosphine (TCEP), and 4 mM MgCl2 for the apoform crystals, and the same composition with 2 mM ADP for the complex crystals, at room temperature, X-ray diffraction structrue determination and analysis at 2.0-2.2 A resolution, molecular replacement and model building using the PDB ID 3FI8 structure as starting model

Plasmodium falciparum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, enzyme binding structure analysis. The ADP molecule also participates in the coordination of two magnesium ions	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + choline	Plasmodium falciparum	-	ADP + phosphocholine	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q8IM71	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography and gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + choline	-	Plasmodium falciparum	ADP + phosphocholine	-	?

Synonyms

Synonyms	Comment	Organism
PfChoK	-	Plasmodium falciparum

General Information

General Information	Comment	Organism
malfunction	selective inhibition of the enzyme and the Kennedy pathway leads to the parasite arrest and eradication	Plasmodium falciparum
metabolism	within the Kennedy pathway, the Plasmodium falciparum choline kinase (PfChoK) has a central role in the biosynthesis of phosphatidylcholine. Plasmodium falciparum plasma membrane is mainly composed by phosphatidylcholine (PC) and phosphatidylethanolamine (PE), which represent 40-50% and 35-40% of the total phospholipid content, respectively	Plasmodium falciparum
additional information	substrate binding structure analysis, overview	Plasmodium falciparum