Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Acetylcholine | stimulates | Rattus norvegicus | |
putrescine | slight stimulation | Rattus norvegicus | |
spermidine | stimulates | Rattus norvegicus | |
spermine | stimulates | Rattus norvegicus |
General Stability | Organism |
---|---|
very stable, even at a low protein concentration, 0.001 mg/ml | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
0.022 | - |
N,N-dimethylethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
0.025 | - |
N,N-diethylethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
0.118 | - |
N-Monomethylethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
0.208 | - |
N,N-Dimethylaminopropanol | 30°C, pH 8.0 | Rattus norvegicus | |
0.4 | - |
N,N-Diisopropylethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
0.444 | - |
beta-Methylcholine | 30°C, pH 8.0 | Rattus norvegicus | |
0.787 | - |
ethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
1 | - |
ATP | 30°C, pH 8.0 | Rattus norvegicus | |
1.2 | - |
ethanolamine | 30°C, pH 8.0 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Carpronium chloride | stimulates | Rattus norvegicus | |
Chlorocholine chloride | stimulates | Rattus norvegicus | |
K+ | stimulates | Rattus norvegicus | |
Li+ | stimulates | Rattus norvegicus | |
Mg2+ | MgATP2- is the true substrate | Rattus norvegicus | |
Mg2+ | stimulates most efficiently of divalent cations | Rattus norvegicus | |
Mg2+ | maximal activity at equimolar concentration of ATP and Mg2+ | Rattus norvegicus | |
Mn2+ | one-sixth as effective as Mg2+ | Rattus norvegicus | |
additional information | Co2+, Cd2+: almost completely ineffective, Ca2+, Ni2+, Zn2+: totally ineffective | Rattus norvegicus | |
Na+ | stimulates | Rattus norvegicus | |
NH4+ | stimulates | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, brain enzyme, SDS-PAGE | Rattus norvegicus |
87600 | - |
sucrose density gradient centrifugation, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.4 | - |
30°C, pH 8.0 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | - |
Rattus norvegicus | ADP + O-phosphocholine | - |
? | |
beta-methylcholine + ATP | - |
Rattus norvegicus | ADP + beta-methylcholine phosphate | - |
? | |
ethanolamine + ATP | - |
Rattus norvegicus | ADP + ethanolamine phosphate | - |
? | |
N,N-diethylethanolamine + ATP | - |
Rattus norvegicus | ADP + N,N-diethylethanolamine phosphate | - |
? | |
N,N-diisopropylethanolamine + ATP | - |
Rattus norvegicus | ADP + N,N-diisopropylethanolamine phosphate | - |
? | |
N,N-dimethylaminopropanol + ATP | - |
Rattus norvegicus | ADP + N,N-dimethylaminopropyl phosphate | - |
? | |
N,N-dimethylethanolamine + ATP | - |
Rattus norvegicus | ADP + N,N-dimethylethanolamine phosphate | - |
? | |
N-methylethanolamine + ATP | - |
Rattus norvegicus | ADP + N-methylethanolamine phosphate | - |
? | |
N-monomethylethanolamine + ATP | - |
Rattus norvegicus | ADP + N-monomethylethanolamine phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, brain enzyme, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
increase of activity up to | Rattus norvegicus |