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Literature summary for 2.7.1.31 extracted from

  • Kleczkowski, L.A.; Randall, D.D.; Zahler, W.L.
    The substrate specificity, kinetics, and mechanism of glycerate-3-kinase from spinach leaves (1985), Arch. Biochem. Biophys., 236, 185-194.
    View publication on PubMed
Search for more literature for 2.7.1.31

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate
-
 Spinacia oleracea
Ribulose 1,5-bisphosphate
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 ATP 25°C Spinacia oleracea
0.25
-
 D-glycerate 25°C Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ forms a complex with ATP Spinacia oleracea
Mn2+ forms a complex with ATP Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
ATP + D-glycerate = ADP + 3-phospho-D-glycerate sequential random mechanism Spinacia oleracea
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate specific for D-isomer Spinacia oleracea ADP + 3-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate equilibrium constant is 303 Spinacia oleracea ADP + 3-phospho-(R)-glycerate
-
 ?
GTP + (R)-glycerate at 42% of the activity with ATP Spinacia oleracea GDP + 3-phospho-(R)-glycerate
-
 ?
UTP + (R)-glycerate at 57% of the activity with ATP Spinacia oleracea UDP + 3-phospho-(R)-glycerate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
200
-
 D-glycerate
-
 Spinacia oleracea