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Literature summary for 2.7.1.31 extracted from

  • Doughty, C.C.; Hayashi, J.A.; Guenther, H.L.
    Purification and properties of D-glycerate 3-kinase from Escherichia coli (1966), J. Biol. Chem., 241, 568-572.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Escherichia coli
iodoacetate
-
Escherichia coli
additional information 60% inhibition by antisera prepared in rabbits against crude extracts of glycerate-grown cells; not: NaF Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
Co2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
Fe2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
Mg2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli
Mn2+ divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
Crook's strain, ATCC 8739
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
210
-
pH 7.4 Escherichia coli

Storage Stability

Storage Stability Organism
-40°C, stable for more than 2 years Escherichia coli
4°C, highly purified crystalline enzyme, 45% ethanol, stable for several weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate
-
Escherichia coli ADP + 3-phospho-(R)-glycerate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
stable Escherichia coli
10
-
stable below Escherichia coli