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Literature summary for 2.7.1.30 extracted from

  • Fukuda, Y.; Abe, A.; Tamura, T.; Kishimoto, T.; Sogabe, A.; Akanuma, S.; Yokobori, S.; Yamagishi, A.; Imada, K.; Inagaki, K.
    Epistasis effects of multiple ancestral-consensus amino acid substitutions on the thermal stability of glycerol kinase from Cellulomonas sp. NT3060 (2016), J. Biosci. Bioeng., 121, 497-502.
    View publication on PubMed
Search for more literature for 2.7.1.30

Protein Variants

Protein Variants Comment Organism
A344V the mutant shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
A344V/T386I/F388Y the mutant with decreased catalytic efficiency shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
C292A the mutant shows a lower melting temperature than the wild type enzyme Cellulomonas sp.
L274M the mutant shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
L274M/T386I/F388Y the mutant shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
Q50E/T386I/F388Y the mutant shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
T386I the mutant shows a higher melting temperature than the wild type enzyme Cellulomonas sp.
T386I/F388Y the mutant with decreased catalytic efficiency shows a 9°C higher melting temperature than the wild type enzyme Cellulomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 glycerol wild type enzyme, at pH 7.5 and 37°C Cellulomonas sp.
0.049
-
 glycerol mutant enzyme T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.
0.087
-
 glycerol mutant enzyme A344V/T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cellulomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycerol Cellulomonas sp.
-
 ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol Cellulomonas sp. NT3060
-
 ADP + sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulomonas sp. D0VZG4
-
-
Cellulomonas sp. NT3060 D0VZG4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
 Cellulomonas sp. ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol
-
 Cellulomonas sp. NT3060 ADP + sn-glycerol 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP: glycerol 3-phosphotransferase
-
 Cellulomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53 60 the melting temperature is 53°C, and the half-life at 60°C is 4 min Cellulomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2500
-
 glycerol mutant enzyme A344V/T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.
2600
-
 glycerol mutant enzyme T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.
4900
-
 glycerol wild type enzyme, at pH 7.5 and 37°C Cellulomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29000
-
 glycerol mutant enzyme A344V/T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.
53000
-
 glycerol mutant enzyme T386I/F388Y, at pH 7.5 and 37°C Cellulomonas sp.
136000
-
 glycerol wild type enzyme, at pH 7.5 and 37°C Cellulomonas sp.