Literature summary for 2.7.1.30 extracted from

Restiawaty, E.; Honda, K.; Okano, K.; Hirota, R.; Omasa, T.; Kuroda, A.; Ohtake, H.
Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions (2012), J. Biosci. Bioeng., 113, 521-525.

Search for more literature for 2.7.1.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells	Thermococcus kodakarensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermococcus kodakarensis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2	-	at pH 7.0 and 70°C	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + glycerol	-	Thermococcus kodakarensis	ADP + sn-glycerol 3-phosphate	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	when the enzyme fused with either polyphosphate kinase of Thermus thermophilus HB27 or an Escherichia coli membrane-intrinsic protein YedZ is incubated at 70°C for 2 h, more than 80% of enzyme activity is retained in the heated Escherichia coli cells	Thermococcus kodakarensis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thermococcus kodakarensis

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Release 2023.1 (January 2023)