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Literature summary for 2.7.1.3 extracted from

  • Asipu, A.; Hayward, B.E.; O'Reilly, J.; Bonthron, D.T.
    Properties of normal and mutant recombinant human ketohexokinases and implications for the pathogenesis of essential fructosuria (2003), Diabetes, 52, 2426-2432.
    View publication on PubMed
Search for more literature for 2.7.1.3

Protein Variants

Protein Variants Comment Organism
A43T decreased thermostability of ketohexokinase-A and ketohexokinase-C Homo sapiens
G40R both ketohexokinase-A and ketohexokinase-C become inactive and largely unsoluble Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 ATP 25°C, pH 7, ketohexokinase-C wild type Homo sapiens
0.15
-
 ATP ATP in form of MgATP2- Homo sapiens
0.32
-
 ATP 25°C, pH 7, ketohexokinase-C A43T Homo sapiens
0.32
-
 ATP ATP in form of MgATP2- Homo sapiens
0.36
-
 ATP 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
0.36
-
 ATP ATP in form of MgATP2- Homo sapiens
0.41
-
 ATP 25°C, pH 7, ketohexokinase-A A43T Homo sapiens
0.41
-
 ATP ATP in form of MgATP2- Homo sapiens
0.44
-
 D-xylose 25°C, pH 7, ketohexokinase-C wild type Homo sapiens
0.8
-
 D-fructose 25°C, pH 7, ketohexokinase-C wild type Homo sapiens
0.84
-
 D-fructose 25°C, pH 7, ketohexokinase-C A43T Homo sapiens
1
-
 D-xylose 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
1.8
-
 D-ribulose 25°C, pH 7, ketohexokinase-C wild type Homo sapiens
5.8
-
 L-sorbose 25°C, pH 7, ketohexokinase-C wild type Homo sapiens
7
-
 D-fructose 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
8.2
-
 D-fructose 25°C, pH 7, ketohexokinase-A A43T Homo sapiens
19.3
-
 L-sorbose 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
32.7
-
 D-ribulose 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
201
-
 D-ribose 25°C, pH 7, ketohexokinase-A wild type Homo sapiens
434
-
 D-ribose 25°C, pH 7, ketohexokinase-C wild type Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Homo sapiens
-
 ADP + D-fructose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Homo sapiens ADP + D-fructose 1-phosphate
-
 ?
ATP + D-ribose
-
 Homo sapiens ?
-
 ?
ATP + D-ribulose
-
 Homo sapiens ?
-
 ?
ATP + D-xylose
-
 Homo sapiens ADP + D-xylose 1-phosphate
-
 ?
ATP + D-xylulose
-
 Homo sapiens ?
-
 ?
ATP + L-sorbose
-
 Homo sapiens ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 C-A43T rapidly loss in activity in absence of glycerol Homo sapiens
55
-
 30 min ketohexokinase-A little loss in activity, ketohexokinase-C completely inactivated Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.55
-
 D-ribose 25°C, pH 7, ketohexokinase-A Homo sapiens
1.7
-
 D-fructose 25°C, pH 7, C-A43T Homo sapiens
4.8
-
 D-ribulose 25°C, pH 7, ketohexokinase-C Homo sapiens
5.2
-
 L-sorbose 25°C, pH 7, ketohexokinase-C Homo sapiens
5.9
-
 D-fructose 25°C, pH 7, A-A43T Homo sapiens
6.5
-
 L-sorbose 25°C, pH 7, ketohexokinase-C Homo sapiens
6.9
-
 D-fructose 25°C, pH 7, ketohexokinase-A Homo sapiens
7.6
-
 D-ribulose 25°C, pH 7, ketohexokinase-A Homo sapiens
7.6
-
 D-fructose 25°C, pH 7, ketohexokinase-C Homo sapiens
7.6
-
 D-xylulose 25°C, pH 7, ketohexokinase-C Homo sapiens
8.2
-
 D-ribose 25°C, pH 7, ketohexokinase-C Homo sapiens
11.1
-
 D-xylulose 25°C, pH 7, ketohexokinase-A Homo sapiens