Cloned (Comment) | Organism |
---|---|
gene dhaK, recombinant expression in Clostridium beijerinckii, coexpression with gene gldA1, encoding a glycerol dehydrogenase (Gldh) from Clostridium pasteurianum | Clostridium pasteurianum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerone | Clostridium pasteurianum | - |
ADP + glycerone phosphate | - |
? | |
ATP + glycerone | Clostridium pasteurianum ATCC 6013 | - |
ADP + glycerone phosphate | - |
? | |
ATP + glycerone | Clostridium pasteurianum DSM 525 | - |
ADP + glycerone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | A0A0H3J0E7 | - |
- |
Clostridium pasteurianum ATCC 6013 | A0A0H3J0E7 | - |
- |
Clostridium pasteurianum DSM 525 | A0A0H3J0E7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerone | - |
Clostridium pasteurianum | ADP + glycerone phosphate | - |
? | |
ATP + glycerone | - |
Clostridium pasteurianum ATCC 6013 | ADP + glycerone phosphate | - |
? | |
ATP + glycerone | - |
Clostridium pasteurianum DSM 525 | ADP + glycerone phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DhaK | - |
Clostridium pasteurianum |