Crystallization (Comment) | Organism |
---|---|
apoenzyme, from solution containing 80 mM sodium acetate, pH 5.0, 160 mM ammonium sulfate, 17% w/v PEG 4000, 15% w/v methylpentanediol, hanging drop vapour diffusion method, apoenzyme-crystals are soaked in 2 mM glyceraldehyde or 5 mM dihydroxyacetone phosphate and flash frozen at -168°C, X-ray diffraction structure determination and analysis at 2.0 and 1.9 A resolution, respectively | Escherichia coli |
General Stability | Organism |
---|---|
enzyme-D,L-glyceraldehyde complex is resistant to SDS | Citrobacter freundii |
not affected by 2 M glycerol | Citrobacter freundii |
not affected by 2 M glycerol | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive to ATP, noncompetitive to D,L-glyceraldehyde | Citrobacter freundii | |
chloro-3-hydroxyacetone | binds to the active site | Citrobacter freundii | |
chloro-3-hydroxyacetone | binds to the active site | Escherichia coli | |
D,L-glyceraldehyde | competitive | Citrobacter freundii | |
D,L-glyceraldehyde | competitive | Escherichia coli | |
additional information | glycerol, hydroxyacetone, hydroxypuruvic acid, and methylglyoxal are no inhibitors | Citrobacter freundii | |
additional information | no inhibition by glycerol, hydroxyacetone, hydroxypuruvic acid, and methylglyoxal | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate binding constants and reaction kinetics, overview | Citrobacter freundii | |
additional information | - |
additional information | substrate binding constants and reaction kinetics, overview | Escherichia coli | |
0.005 | - |
glycerone phosphate | or below, pH 7.5, 30°C | Citrobacter freundii | |
0.006 | - |
glycerone phosphate | pH 7.5, 30°C | Escherichia coli | |
0.1 | - |
ATP | pH 7.5, 30°C | Citrobacter freundii | |
0.18 | - |
D,L-glyceraldehyde | pH 7.5, 30°C | Citrobacter freundii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycerone | Citrobacter freundii | - |
ADP + glycerone phosphate | - |
? | |
phosphoenolpyruvate + glycerone | Escherichia coli | - |
pyruvate + glycerone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Escherichia coli | P37349 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + glycerone = ADP + glycerone phosphate | covalent substrate-binding and random bi bi kinetic mechanism | Citrobacter freundii | |
ATP + glycerone = ADP + glycerone phosphate | substrates and products are bound in hemiaminal linkage to the active site histidine, thereby not activating the cataltically reacting hydroxyl group | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + DL-glyceraldehyde | D,L-glyceraldehyde binds strongly to the enzyme, slow product release | Citrobacter freundii | ADP + DL-glyceraldehyde 3-phosphate | - |
? | |
ATP + glycerone | - |
Citrobacter freundii | ADP + glycerone phosphate | - |
? | |
ATP + glycerone | - |
Citrobacter freundii | ADP + glycerone phosphate | i.e. dihydroxyacetone phosphate | ? | |
additional information | no activity with glycerol, hydroxyacetone, hydroxypuruvic acid, chloro-3-hydroxyacetone, and methylglyoxal | Escherichia coli | ? | - |
? | |
additional information | no activity with glycerol, hydroxyacetone, hydroxypyruvic acid, chloro-3-hydroxyacetone, and methylglyoxal | Citrobacter freundii | ? | - |
? | |
phosphoenolpyruvate + DL-glyceraldehyde | - |
Escherichia coli | pyruvate + DL-glyceraldehyde 3-phosphate | - |
? | |
phosphoenolpyruvate + glycerone | - |
Escherichia coli | pyruvate + glycerone phosphate | - |
? | |
phosphoenolpyruvate + glycerone | - |
Escherichia coli | pyruvate + glycerone phosphate | i.e. dihydroxyacetone phosphate | ? |
Subunits | Comment | Organism |
---|---|---|
trimer | enzyme exists of a small, a large, and a substrate binding subunit | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHA kinase | - |
Citrobacter freundii |
DHA kinase | - |
Escherichia coli |
DhaK | - |
Citrobacter freundii |
DhaK | - |
Escherichia coli |
dihydroxyacetone kinase | - |
Citrobacter freundii |
dihydroxyacetone kinase | - |
Escherichia coli |
More | dihydroxyacetoe phosphate kinases exist in 2 different groups, one utilizing ATP as phosphate donor, the other utilizing phosphoenolpyrivate | Escherichia coli |
More | dihydroxyacetone phosphate kinases exist in 2 different groups, one utilizing ATP as phosphate donor, the other utilizing phosphoenolpyrivate | Citrobacter freundii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
phosphotransferase assay at | Citrobacter freundii |
30 | - |
phosphotransferase assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
glycerone phosphate | pH 7.5, 30°C | Escherichia coli | |
5.17 | - |
D,L-glyceraldehyde | pH 7.5, 30°C | Citrobacter freundii | |
17.5 | - |
glycerone phosphate | pH 7.5, 30°C | Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
phosphotransferase assay at | Citrobacter freundii |
7.5 | - |
phosphotransferase assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Citrobacter freundii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Citrobacter freundii | |
additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
0.14 | - |
ADP | pH 7.5, 30°C | Citrobacter freundii | |
0.15 | - |
D,L-glyceraldehyde | pH 7.5, 30°C | Escherichia coli | |
0.6 | - |
D,L-glyceraldehyde | pH 7.5, 30°C | Citrobacter freundii |