Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | free ADP, 0.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde | Rattus norvegicus | |
ATP | free ATP, 1.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde | Rattus norvegicus | |
MgADP- | free, competitive to MgATP2-, noncompetitive to D-glyceraldehyde | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
dihydroxyacetone | pH 7.5, 23-24°C, at 2 mM ATPMg2- | Rattus norvegicus | |
0.008 | - |
D-glyceraldehyde | pH 7.5, 23-24°C, cosubstrate 1.0 mM MgATP2- | Rattus norvegicus | |
0.012 | - |
D-glyceraldehyde | pH 7.5, 23-24°C, cosubstrate 0.5 mM MgATP2- | Rattus norvegicus | |
0.019 | - |
D-glyceraldehyde | pH 7.5, 23-24°C, cosubstrate 2.0 mM MgATP2- | Rattus norvegicus | |
0.16 | - |
ATP | pH 7.5, 23-24°C, 11.2 mM free Mg2+ | Rattus norvegicus | |
0.16 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.2 | - |
ATP | pH 7.5, 23-24°C, 1.11 mM free Mg2+ | Rattus norvegicus | |
0.2 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.24 | - |
ATP | pH 7.5, 23-24°C, 0.34 mM free Mg2+ | Rattus norvegicus | |
0.24 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required in equimolar concentration with ATP | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glyceraldehyde | - |
Rattus norvegicus | ADP + D-glyceraldehyde 3-phosphate | - |
? | |
ATP + D-glyceraldehyde | - |
Rattus norvegicus | ADP + D-glyceraldehyde-3-phosphate | - |
? | |
ATP + dihydroxyacetone | 2.5fold higher activity than with D-glyceraldehyde | Rattus norvegicus | ADP + dihydroxyacetone phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
ATP | pH 7.5, 23-24°C | Rattus norvegicus | |
1.1 | - |
ADP | pH 7.5, 23-24°C | Rattus norvegicus |