General Stability | Organism |
---|---|
enzyme is inactivated by freezing and thawing unless both riboflavin and 20% glycerol are added | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Deazariboflavin | - |
Rattus norvegicus | |
10-(5'-Hydroxypentyl)flavin | - |
Rattus norvegicus | |
10-(Hydroxyethyl)flavin | - |
Rattus norvegicus | |
3-Deazariboflavin | - |
Rattus norvegicus | |
5-Deazariboflavin | - |
Rattus norvegicus | |
8-Aminoriboflavin | - |
Rattus norvegicus | |
8-Diethylaminoriboflavin | - |
Rattus norvegicus | |
8-Ethoxyriboflavin | - |
Rattus norvegicus | |
8-Ethylaminoriboflavin | - |
Rattus norvegicus | |
8-hydroxyriboflavin | - |
Rattus norvegicus | |
8-Methoxyriboflavin | - |
Rattus norvegicus | |
8-Methylaminoriboflavin | - |
Rattus norvegicus | |
8-Methylethylaminoriboflavin | - |
Rattus norvegicus | |
Ba2+ | - |
Rattus norvegicus | |
Fe2+ | - |
Rattus norvegicus | |
lumichrome | - |
Rattus norvegicus | |
Lumiflavin | - |
Rattus norvegicus | |
riboflavin 5'-phosphate | - |
Rattus norvegicus | |
Sr2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
riboflavin | pH 8.0, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Rattus norvegicus | |
Zn2+ | activation is 1.8times of that with Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Rattus norvegicus |
28000 | - |
1 * 28000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.165 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity after 2 d when the enzyme is stored in buffer alone, complete protection by 0.01 mM riboflavin | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 10-(D-allo)flavin | 30% of the activity with riboflavin | Rattus norvegicus | ADP + 10-(D-allo)flavin 5'-phosphate | - |
? | |
ATP + 10-(L-arabo)flavin | 25% of the activity with riboflavin | Rattus norvegicus | ADP + 10-(L-arabo)flavin 5'-phosphate | - |
? | |
ATP + 2-thioriboflavin | 60% of the activity with riboflavin | Rattus norvegicus | ADP + 2-thioriboflavin 5'-phosphate | - |
? | |
ATP + 8-demethylriboflavin | 35% of the activity with riboflavin | Rattus norvegicus | ADP + 8-demethylriboflavin 5'-phosphate | - |
? | |
ATP + 8-dimethylamino-8-demethylriboflavin | 70% of the activity with riboflavin | Rattus norvegicus | ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate | - |
? | |
ATP + D-erythroflavin | 10-(D-erythro)flavin, 33% of the activity with riboflavin | Rattus norvegicus | ADP + D-erythroflavin 5'-phosphate | - |
? | |
ATP + riboflavin | - |
Rattus norvegicus | ADP + FMN | - |
? | |
dATP + riboflavin | - |
Rattus norvegicus | dADP + FMN | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Lumiflavin | pH 8, 37°C | Rattus norvegicus | |
0.007 | - |
10-(Hydroxyethyl)flavin | pH 8, 37°C | Rattus norvegicus | |
0.008 | - |
10-(5'-Hydroxypentyl)flavin | pH 8, 37°C | Rattus norvegicus | |
0.01 | - |
lumichrome | pH 8, 37°C | Rattus norvegicus | |
0.015 | - |
8-Methoxyriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.016 | - |
8-Ethoxyriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.018 | - |
riboflavin 5'-phosphate | pH 8, 37°C | Rattus norvegicus | |
0.02 | - |
8-Diethylaminoriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.021 | - |
1-Deazariboflavin | pH 8, 37°C | Rattus norvegicus | |
0.03 | - |
8-Methylethylaminoriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.175 | - |
8-Ethylaminoriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.25 | - |
8-Aminoriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.275 | - |
5-Deazariboflavin | pH 8, 37°C | Rattus norvegicus | |
0.41 | - |
3-Deazariboflavin | pH 8, 37°C | Rattus norvegicus | |
0.47 | - |
8-Methylaminoriboflavin | pH 8, 37°C | Rattus norvegicus | |
0.5 | - |
8-hydroxyriboflavin | pH 8, 37°C | Rattus norvegicus |