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Literature summary for 2.7.1.26 extracted from

  • Merrill, A.H.; McCormick, D.B.
    Affinity chromatographic purification and properties of flavokinase (ATP:riboflavin 5-phosphotransferase) from rat liver (1980), J. Biol. Chem., 255, 1335-1338.
    View publication on PubMed

General Stability

General Stability Organism
enzyme is inactivated by freezing and thawing unless both riboflavin and 20% glycerol are added Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1-Deazariboflavin
-
Rattus norvegicus
10-(5'-Hydroxypentyl)flavin
-
Rattus norvegicus
10-(Hydroxyethyl)flavin
-
Rattus norvegicus
3-Deazariboflavin
-
Rattus norvegicus
5-Deazariboflavin
-
Rattus norvegicus
8-Aminoriboflavin
-
Rattus norvegicus
8-Diethylaminoriboflavin
-
Rattus norvegicus
8-Ethoxyriboflavin
-
Rattus norvegicus
8-Ethylaminoriboflavin
-
Rattus norvegicus
8-hydroxyriboflavin
-
Rattus norvegicus
8-Methoxyriboflavin
-
Rattus norvegicus
8-Methylaminoriboflavin
-
Rattus norvegicus
8-Methylethylaminoriboflavin
-
Rattus norvegicus
Ba2+
-
Rattus norvegicus
Fe2+
-
Rattus norvegicus
lumichrome
-
Rattus norvegicus
Lumiflavin
-
Rattus norvegicus
riboflavin 5'-phosphate
-
Rattus norvegicus
Sr2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
riboflavin pH 8.0, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Rattus norvegicus
Zn2+ activation is 1.8times of that with Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Rattus norvegicus
28000
-
1 * 28000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.165
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 2 d when the enzyme is stored in buffer alone, complete protection by 0.01 mM riboflavin Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 10-(D-allo)flavin 30% of the activity with riboflavin Rattus norvegicus ADP + 10-(D-allo)flavin 5'-phosphate
-
?
ATP + 10-(L-arabo)flavin 25% of the activity with riboflavin Rattus norvegicus ADP + 10-(L-arabo)flavin 5'-phosphate
-
?
ATP + 2-thioriboflavin 60% of the activity with riboflavin Rattus norvegicus ADP + 2-thioriboflavin 5'-phosphate
-
?
ATP + 8-demethylriboflavin 35% of the activity with riboflavin Rattus norvegicus ADP + 8-demethylriboflavin 5'-phosphate
-
?
ATP + 8-dimethylamino-8-demethylriboflavin 70% of the activity with riboflavin Rattus norvegicus ADP + 8-dimethylamino-8-demethylriboflavin 5'-phosphate
-
?
ATP + D-erythroflavin 10-(D-erythro)flavin, 33% of the activity with riboflavin Rattus norvegicus ADP + D-erythroflavin 5'-phosphate
-
?
ATP + riboflavin
-
Rattus norvegicus ADP + FMN
-
?
dATP + riboflavin
-
Rattus norvegicus dADP + FMN
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
Lumiflavin pH 8, 37°C Rattus norvegicus
0.007
-
10-(Hydroxyethyl)flavin pH 8, 37°C Rattus norvegicus
0.008
-
10-(5'-Hydroxypentyl)flavin pH 8, 37°C Rattus norvegicus
0.01
-
lumichrome pH 8, 37°C Rattus norvegicus
0.015
-
8-Methoxyriboflavin pH 8, 37°C Rattus norvegicus
0.016
-
8-Ethoxyriboflavin pH 8, 37°C Rattus norvegicus
0.018
-
riboflavin 5'-phosphate pH 8, 37°C Rattus norvegicus
0.02
-
8-Diethylaminoriboflavin pH 8, 37°C Rattus norvegicus
0.021
-
1-Deazariboflavin pH 8, 37°C Rattus norvegicus
0.03
-
8-Methylethylaminoriboflavin pH 8, 37°C Rattus norvegicus
0.175
-
8-Ethylaminoriboflavin pH 8, 37°C Rattus norvegicus
0.25
-
8-Aminoriboflavin pH 8, 37°C Rattus norvegicus
0.275
-
5-Deazariboflavin pH 8, 37°C Rattus norvegicus
0.41
-
3-Deazariboflavin pH 8, 37°C Rattus norvegicus
0.47
-
8-Methylaminoriboflavin pH 8, 37°C Rattus norvegicus
0.5
-
8-hydroxyriboflavin pH 8, 37°C Rattus norvegicus