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Literature summary for 2.7.1.25 extracted from

  • De-zhen, W.; Guo-guo, C.; Lu-jia, L.; Zhao-jun, J.; Yu-chun, R.; Mei-hao, S.
    In vitro functional study of rice adenosine 5'-phosphosulfate kinase (2016), Rice Sci., 23, 152-159.
No PubMed abstract available
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Protein Variants

Protein Variants Comment Organism
C36A/C69A the mutant is not inhibited by oxidized glutathione and shows activity reduced to 71% Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-phosphosulfate
-
 Oryza sativa
ATP
-
 Oryza sativa
oxidized glutathione
-
 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
 adenosine-5'-phosphosulfate mutant enzyme C36A/C69A, at pH 8.0 and 25°C Oryza sativa
0.00072
-
 adenosine-5'-phosphosulfate wild type enzyme, at pH 8.0 and 25°C Oryza sativa
0.14
-
 ATP mutant enzyme C36A/C69A, at pH 8.0 and 25°C Oryza sativa
0.75
-
 ATP wild type enzyme, at pH 8.0 and 25°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine-5'-phosphosulfate Oryza sativa
-
 ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 variety Nipponbare
-

Purification (Commentary)

Purification (Comment) Organism
PAP-agarose column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-
root
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine-5'-phosphosulfate
-
 Oryza sativa ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulfate kinase
-
 Oryza sativa
APS kinase
-
 Oryza sativa
APSK1
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.17
-
 adenosine-5'-phosphosulfate mutant enzyme C36A/C69A, at pH 8.0 and 25°C Oryza sativa
5
-
 adenosine-5'-phosphosulfate wild type enzyme, at pH 8.0 and 25°C Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00058
-
 adenosine-5'-phosphosulfate wild type enzyme, at pH 8.0 and 25°C Oryza sativa
0.61
-
 ATP wild type enzyme, at pH 8.0 and 25°C Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4917
-
 adenosine-5'-phosphosulfate mutant enzyme C36A/C69A, at pH 8.0 and 25°C Oryza sativa
6950
-
 adenosine-5'-phosphosulfate wild type enzyme, at pH 8.0 and 25°C Oryza sativa