Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.25 extracted from

  • Herrmann, J.; Nathin, D.; Lee, S.G.; Sun, T.; Jez, J.M.
    Recapitulating the structural evolution of redox regulation in adenosine 5'-phosphosulfate kinase from cyanobacteria to Plants (2015), J. Biol. Chem., 290, 24705-24714.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with AMP-PNP and adenosine-5'-phosphosulfate, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate, pH 6.5, 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) PEG 8000 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
H23C the mutation does not significantly alter the steady-state kinetic parameters of the enzyme Synechocystis sp.
T61E the mutation does not significantly alter the steady-state kinetic parameters of the enzyme Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-phosphosulfate substrate inhibition Synechocystis sp.
sulfate
-
 Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 2 * 20000, SDS-PAGE Synechocystis sp.
40000
-
 gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine-5'-phosphosulfate Synechocystis sp.
-
 ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P72940
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Ni-NTA/benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine-5'-phosphosulfate
-
 Synechocystis sp. ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 20000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulfate kinase
-
 Synechocystis sp.
APS kinase
-
 Synechocystis sp.
APSK
-
 Synechocystis sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
15.7
-
 mutant enzyme T61E, pH and temperature not specified in the publication Synechocystis sp. sulfate
17
-
 wild type enzyme, pH and temperature not specified in the publication Synechocystis sp. sulfate