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Literature summary for 2.7.1.25 extracted from

  • Ravilious, G.E.; Nguyen, A.; Francois, J.A.; Jez, J.M.
    Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase (2012), Proc. Natl. Acad. Sci. USA, 109, 309-314.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
APSK isoform 1 in complex with beta,gamma-imidoadenosine-5'-triphosphate, Mg2+, and adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 200 mM MgCl2, and 15-17.5% (w/v) PEG 2000 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C86A/C119A the mutant is kinetically similar to the reduced wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate
-
Arabidopsis thaliana
adenosine 5'-phosphosulfate
-
Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.00048
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.00087
-
adenosine 5'-phosphosulfate oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
0.0019
-
adenosine 5'-phosphosulfate reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q43295
-
-
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex-200 gel filtration Synechocystis sp.
Ni-NTA column chromatography and Superdex-200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
Synechocystis sp. ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
ATP + adenosine 5'-phosphosulfate
-
Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Synonyms

Synonyms Comment Organism
adenosine-5'-phosphosulfate kinase
-
Arabidopsis thaliana
adenosine-5’-phosphosulfate kinase
-
Synechocystis sp.
APSK
-
Synechocystis sp.
APSK
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.1
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
203
-
adenosine 5'-phosphosulfate oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
239
-
adenosine 5'-phosphosulfate reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
272
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00251
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.0348
-
adenosine 5'-phosphosulfate reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
0.0375
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.0416
-
adenosine 5'-phosphosulfate oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function in Arabidopsis thaliana, the enzyme is essential for reproductive viability Synechocystis sp.
physiological function in Arabidopsis thaliana, the enzyme is essential for reproductive viability Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
32800
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
125000
-
adenosine 5'-phosphosulfate reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
233000
-
adenosine 5'-phosphosulfate oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C Arabidopsis thaliana
567000
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana