Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Synechocystis sp. |
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
APSK isoform 1 in complex with beta,gamma-imidoadenosine-5'-triphosphate, Mg2+, and adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 200 mM MgCl2, and 15-17.5% (w/v) PEG 2000 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C86A/C119A | the mutant is kinetically similar to the reduced wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana | |
adenosine 5'-phosphosulfate | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.00048 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.00087 | - |
adenosine 5'-phosphosulfate | oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0019 | - |
adenosine 5'-phosphosulfate | reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43295 | - |
- |
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex-200 gel filtration | Synechocystis sp. |
Ni-NTA column chromatography and Superdex-200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Synechocystis sp. | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
ATP + adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosine-5'-phosphosulfate kinase | - |
Arabidopsis thaliana |
adenosine-5-phosphosulfate kinase | - |
Synechocystis sp. |
APSK | - |
Synechocystis sp. |
APSK | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.1 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
203 | - |
adenosine 5'-phosphosulfate | oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
239 | - |
adenosine 5'-phosphosulfate | reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
272 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00251 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0348 | - |
adenosine 5'-phosphosulfate | reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0375 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0416 | - |
adenosine 5'-phosphosulfate | oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | in Arabidopsis thaliana, the enzyme is essential for reproductive viability | Synechocystis sp. |
physiological function | in Arabidopsis thaliana, the enzyme is essential for reproductive viability | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32800 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
125000 | - |
adenosine 5'-phosphosulfate | reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
233000 | - |
adenosine 5'-phosphosulfate | oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
567000 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |