Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D87A | almost complete loss of activity | Mus musculus |
D87E | 80% loss of activity | Mus musculus |
D87R | almost complete loss of activity | Mus musculus |
D89A | almost complete loss of activity | Mus musculus |
D89E | 75% loss of activity | Mus musculus |
D89R | almost complete loss of activity | Mus musculus |
DN89ND | switch mutant, almost complete loss of activity | Mus musculus |
G88A | less than 30% loss of kinase activity | Mus musculus |
G88D | almost complete loss of activity | Mus musculus |
G88R | almost complete loss of activity | Mus musculus |
K97A | no effect on activity | Mus musculus |
LD86DL | switch mutant, almost complete loss of activity | Mus musculus |
N90A | 30% loss of kinase activity | Mus musculus |
N90Q | 30% loss of kinase activity | Mus musculus |
R92A | complete loss of kinase activity | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | Mus musculus | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000298 | - |
wild type activity, specific activities given for all mutant enzymes | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Mus musculus | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? | |
ATP + adenosine 5'-phosphosulfate | synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes | Mus musculus | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate | Mus musculus |