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Literature summary for 2.7.1.25 extracted from

  • Lansdon, E.B.; Fisher, A.J.; Segel, I.H.
    Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains (2004), Biochemistry, 43, 4356-4365.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate substrate inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens
0.14
-
ATP pH 8.0, 30°C Homo sapiens
0.14
-
ATP ATP in form of MgATP2- Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in complex with ATP Homo sapiens
NaCl increased activity at high salt conditions, 100 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
2 * 71000 Homo sapiens
150000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine 5'-phosphosulfate Homo sapiens synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43252 isoform 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
pH 8.0. 30°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?
ATP + adenylyl sulfate
-
Homo sapiens ADP + 3'-phosphoadenylyl sulfate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 71000 Homo sapiens

Synonyms

Synonyms Comment Organism
More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.048
-
adenosine 5'-phosphosulfate pH 8.0, 30°C Homo sapiens