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Literature summary for 2.7.1.25 extracted from

  • Harjes, S.; Scheidig, A.; Bayer, P.
    Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1 (2004), Acta Crystallogr. Sect. D, 60, 350-352.
    View publication on PubMed
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
162000
-
 gel filtration Homo sapiens
716000
-
 2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine 5'-phosphosulfate Homo sapiens synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
 Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?
ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens
PAPS synthetase two isoforms, type 1 and 2 Homo sapiens